PXD029628

PXD029628 is an original dataset announced via ProteomeXchange.

Title	Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and their adaptation to different salinity probed by a proteomic approach
Description	Osmotic changes are common challenges for marine microorganisms. Bacteria developed numerous ways of dealing with this stress, including reprogramming of global cellular processes, however, many molecular details were obtained only for the model bacteria. In this work we asked what is the basis of the adjustment to prolonged salinity challenges at the proteome level. The objects of our studies were three representatives of bacteria inhabiting various marine environments, Shewanella baltica, Vibrio harveyi and Aliivibrio fischeri. The proteomic studies were performed with bacteria cultivated in increased and decreased salinity, followed by proteolytic digestion of samples which were then subjected to liquid chromatography with tandem mass spectrometry analysis. We show that bacteria adjust at all levels of their biological processes, from DNA topology through gene expression regulation and proteasome assembly, to transport and cellular metabolism. Finding that many similar adaptation strategies were observed for both, low and high salinity conditions, is particularly interesting. The results show that adaptation to salinity challenge involves accumulation of DNA-binding proteins and increased polyamine uptake, and we hypothesize that their function is to coat and protect the nucleoid to counteract adverse changes in the DNA topology due to ionic shifts.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_03:05:37.284.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Paulina Czaplewska
SpeciesList	scientific name: Aliivibrio fischeri; NCBI TaxID: 668; scientific name: Shewanella baltica; NCBI TaxID: 62322; scientific name: Vibrio harveyi; NCBI TaxID: 669;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600+

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-09 05:47:21	ID requested
⏵ 1	2022-02-17 03:05:38	announced

Kloska A, Cech GM, Nowicki D, Maci, ą, g-Dorszy, ń, ska M, Bogucka AE, Markert S, Becher D, Potrykus K, Czaplewska P, Szalewska-Pa, ł, asz A, , and Their Adaptation to Different Salinity Probed by a Proteomic Approach. Int J Mol Sci, 23(2):(2022) [pubmed]

submitter keyword: Shewanella baltica, Aliivibrio fischeri, Vibrio harveyi, osmotic stress, salinity challenge, LC-MS/MS

Paulina Czaplewska
contact affiliation	Laboratory of Mass Spectrometry, Core Facility Laboratories, Intercollegiate Faculty of Biotechnology UG&MUG, University of Gdansk, Gdansk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
Paulina Czaplewska
contact affiliation	University of Gdansk
contact email	paulina.czaplewska@ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029628

PRIDE project URI

• PRIDE
  1. PXD029628
     1. Label: PRIDE project
     2. Name: Three Microbial Musketeers of the Seas: Shewanella baltica, Aliivibrio fischeri and Vibrio harveyi, and their adaptation to different salinity probed by a proteomic approach