PXD029624

PXD029624 is an original dataset announced via ProteomeXchange.

Title	Hypocrates, a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives
Description	The lack of tools to monitor the dynamics of (pseudo)hypohalous acids in live cells and tissues hinders a better understanding of inflammatory processes. Here we present a fluorescent genetically encoded biosensor, Hypocrates, for the visualization of (pseudo)hypohalous acids and their derivatives. Hypocrates consists of a circularly permuted yellow fluorescent protein integrated into the structure of the transcription repressor NemR from Escherichia coli. We show that Hypocrates is ratiometric, reversible, and responds to its analytes in the 106 M-1s-1 range. Solving the Hypocrates X-ray structure provided insights into its sensing mechanism, making it the first circularly permuted fluorescent protein-based redox probe for which spatial organization is determined. We exemplify its applicability by imaging hypohalous stress in bacteria phagocytosed by primary neutrophils. Finally, we demonstrate that Hypocrates can be utilized in combination with HyPerRed for the simultaneous visualization of (pseudo)hypohalous acids and hydrogen peroxide dynamics in a zebrafish tail fin injury model
HostingRepository	PRIDE
AnnounceDate	2023-04-11
AnnouncementXML	Submission_2023-04-11_06:29:01.068.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029624
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	DidierVertommen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	dihydroxylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-09 03:06:26	ID requested
1	2021-12-14 23:49:20	announced
⏵ 2	2023-04-11 06:29:01	announced	2023-04-11: Updated project metadata.

10.6019/PXD029624;

Kostyuk AI, Tossounian MA, Panova AS, Thauvin M, Raevskii RI, Ezeri, ņ, a D, Wahni K, Van Molle I, Sergeeva AD, Vertommen D, Gorokhovatsky AY, Baranov MS, Vriz S, Messens J, Bilan DS, Belousov VV, Hypocrates is a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives. Nat Commun, 13(1):171(2022) [pubmed]

submitter keyword: fluorescence,biosensor, visualization, hypohalous acids, ratiometric

Vsevolod V.Belousov
contact affiliation	Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 117997 Moscow, Russia
contact email	belousov@fccps.ru
lab head
DidierVertommen
contact affiliation	UCL - de Duve Institute, Brussels Belgium
contact email	didier.vertommen@uclouvain.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD029624

PRIDE project URI

• PRIDE
  1. PXD029624
     1. Label: PRIDE project
     2. Name: Hypocrates, a genetically encoded fluorescent biosensor for (pseudo)hypohalous acids and their derivatives