PXD029621

PXD029621 is an original dataset announced via ProteomeXchange.

Title	Protein composition of Moorella thermoacetica spores formed at contrasted temperatures
Description	Moorella thermoacetica spores are the most heat-resistant so far retrieved in food industry and we previously showed that the resistance properties of these spores to wet- heat and biocides were lower when spores were produced at low limit temperature than at optimal temperature. By electron microcopy, we observed that the ultrastructure of the spore coat differed according to the sporulation temperature, with spores produced at 55 °C mainly exhibiting lamellar inner coat tightly associated to diffuse outer coat, while spores produced at 45 °C showing an inner and outer coat separated by a less electron- dense zone. Moreover, misarranged coat structures were more frequently observed when spores were produced at low limit temperature. We analyzed the proteome of spore ob- tained at 45° and 55 °C and focused our data analysis on putative spore coat, exosporium proteins or proteins playing a role in spore resistance. Some putative spore coat proteins, such as CotSA, were only identified in spores produced at 55 °C, while some other puta- tive exosporium and coat proteins were significantly less abundant in spores produced at 45 °C. Altogether, our results suggest that sporulation temperature affects the structure and the protein composition of M. thermoacetica spores.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_02:55:13.627.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Celine Henry
SpeciesList	scientific name: Moorella thermoacetica ATCC 39073; NCBI TaxID: 264732;
ModificationList	iodoacetamide derivatized residue
Instrument	Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-08 09:11:09	ID requested
⏵ 1	2022-02-17 02:55:14	announced

Malleck T, Fekraoui F, Bornard I, Henry C, Haudebourg E, Planchon S, Broussolle V, Spores Formed at Different Temperatures. Int J Mol Sci, 23(1):(2022) [pubmed]

submitter keyword: Moorella thermoacetica, spores, LC-MS/MS

Véronique Broussolle
contact affiliation	INRAE, UMR408 Sécurité et Qualité des Produits d'Origine Végétale
contact email	veronique.broussolle@inrae.fr
lab head
Celine Henry
contact affiliation	INRAE
contact email	celine.henry@inra.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029621
     1. Label: PRIDE project
     2. Name: Protein composition of Moorella thermoacetica spores formed at contrasted temperatures