PXD029611

PXD029611 is an original dataset announced via ProteomeXchange.

Title	Profiling of mouse sciatic nerve acetylome
Description	Lysine acetylation is a reversible Post-translational modification (PTM) involved in a broad array of physiological functions. Recent studies have demonstrated the involvement of protein acetylation in modulating Schwann cells' (SCs) biology and the peripheral nervous system (PNS) regeneration. However, the underlying mechanism is still under investigation. This descriptive study characterized the mouse sciatic nerve (SN) acetylome and explored their cellular distribution. Using a modified workflow for acetylome analysis, we identified 483 acetylated proteins containing 1,442 acetylation modification sites in the SN of adult C57BL/6. Notably, over 70% of identified peptides were acetylated. Bioinformatic analysis suggested that these acetylated proteins are mainly located in the myelin sheath, mitochondrial inner membrane, and actin cytoskeleton, and highlighted the remarkable differences between the mouse SN and brain (PXD027299）acetylome. Further manual annotation indicated that most acetylated proteins were mitochondrial and energy, and cytoskeleton and cell adhesion (> 45%). The acetylate modification of three novel myelin-related proteins were verified, including 2', 3' -cycling-nucleotide 3'-phosphodiesterase (CNP), Neurofilament light polypeptide (NEFL), neurofilament medium/high polypeptide (NFM/H), and periaxin (PRX). Immunofluorescence staining illustrated that, besides the nuclei, the acetylated proteins, including acetylated alpha-tubulin, were mainly co-localized with S100 positive SCs. Together, for the first time, this study provided a comprehensive mouse SN acetylome by using a simple but effective method. Moreover, we demonstrated that the acetylated SN proteins were predominantly located in SCs. These analyses and the method will contribute to understanding and uncovering the roles of protein acetylation in SCs development and PNS regeneration.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:52.017.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuhua JI
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-08 02:55:01	ID requested
1	2022-10-14 19:13:09	announced
⏵ 2	2023-11-14 08:53:52	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Acetylome, Sciatic nerve, Mouse,Acetylation, Schwann cells

Yuhua ji
contact affiliation	Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
contact email	tjiyuhua@126.com
lab head
Yuhua JI
contact affiliation	Department of Immunobiology, College of Life Science and Technology, Jinan University, Guangzhou, China.
contact email	tjiyuhua@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029611
     1. Label: PRIDE project
     2. Name: Profiling of mouse sciatic nerve acetylome