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PXD029591

PXD029591 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntact extracellular vesicle cross-linking on vesicles derived from MDA-MB-231 and LM2 cells
DescriptionThe development of any pharmaceutical requires characterization of the compound, usually be several techniques. Therefore, characterizing the protein content and biochemical state of EVs is of great importance for their useif they are to be used as biopharmaceuticals. Structural characterization of proteins in EVs remains a great challenge even for state-of-the-art technologies such as super-resolution and electron microscopy, partly due to the biophysical and biochemical heterogeneity of EVs. Here we overcome some of these challenges by using cross-linking mass spectrometry (XL-MS) to structurally characterize protein complexes and the interactome of intact EVs, at an unprecedented resolution of up to 30Å. By applying XL-MS to intact EVs derived from two metastatic breast cancer cell lines (MDA-MB-231 and LM2), we validate the presence of active forms of protein complexes that are known to play a role metastasis, revealing for example previously uncharacterized regions of Moesin. Moreover, a cross-linking data-driven molecular docking supports novel conformations of ANXA2 in LM2 EVs.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:14:32.020.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJulia Bauzá Martinez
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-05 05:00:36ID requested
12022-11-08 04:59:30announced
22023-11-14 08:14:35announced2023-11-14: Updated project metadata.
Publication List
Bauz, á, -Martinez J, Armony G, Pronker MF, Wu W, Characterization of protein complexes in extracellular vesicles by intact extracellular vesicle crosslinking mass spectrometry (iEVXL). J Extracell Vesicles, 11(8):e12245(2022) [pubmed]
Keyword List
submitter keyword: TNBC,EVs, XL-MS, structural proteomics, CX-MS
Contact List
Wei Wu
contact affiliation1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands 2. Netherlands Proteomics Centre, Padualaan 8, 3584 CH Utrecht, The Netherlands
contact emailw.wu1@uu.nl
lab head
Julia Bauzá Martinez
contact affiliationUtrecht University
contact emailj.bauzamartinez@uu.nl
dataset submitter
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Dataset FTP location
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