PXD029586

PXD029586 is an original dataset announced via ProteomeXchange.

Title	Analysis of protein secretome of human hepatocellular carcinoma (HCC) Cancer Associated Fibroblasts (CAFs) after long term (14 days) treatment with Transforming Growth Factor beta 1 (TGF beta 1)
Description	Cancer-Associated Fibroblasts (CAFs) were isolated from specimens taken from primary human hepatocellular carcinoma (HCC) nodules (4 patients in total), characterized for expression of typical stromal markers (including vimentin and alpha smooth muscle actin), and then incubated in complete medium (Iscove's Modified Dulbecco Medium, IMDM + 20% fetal bovine serum + antibiotic/antimycotic) without any treatment, or in the presence of Transforming Growth Factor Beta 1 (TGF beta 1) at a concentration of 5 ng/ml. The medium and the treatment were renewed every 2 days until the 14th day. At that time point, cells were washed 4 times with serum-free IMDM medium and then incubated with serum free IMDM medium for 48 hours to allow for enrichment with secreted CAFs proteins. The CAFs conditioned medium was then collected, concentrated using a centricon device (10 kDa cutoff), assayed for protein concentration, and finally subjected to Liquid Chromatography with tandem mass spectrometry (LC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2021-11-11
AnnouncementXML	Submission_2021-11-11_00:40:26.411.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francesco Dituri
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-05 05:00:07	ID requested
⏵ 1	2021-11-11 00:40:26	announced

Dataset with its publication pending

submitter keyword: Hepatocellular Carcinoma, Cancer Associated Fibroblasts, TGF beta, Protein Secretome, LC-MS/MS

Francesco Dituri
contact affiliation	National Institute of Gastroenterology, IRCCS "S. De Bellis" Research Hospital, Castellana Grotte, 70013 Bari, Italy
contact email	francesco.dituri@irccsdebellis.it
lab head
Francesco Dituri
contact affiliation	National Institute for Gastroenterology, IRCCS 'S. De Bellis' Research Hospital, 70013, Castellana Grotte (Ba)
contact email	francesco.dituri@irccsdebellis.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029586
     1. Label: PRIDE project
     2. Name: Analysis of protein secretome of human hepatocellular carcinoma (HCC) Cancer Associated Fibroblasts (CAFs) after long term (14 days) treatment with Transforming Growth Factor beta 1 (TGF beta 1)