PXD029584

PXD029584 is an original dataset announced via ProteomeXchange.

Title	Adaptiven mechanism of antibiotic resistant Staphylococus aureus to host environment
Description	Antibiotic-resistant bacteria can escape from the killing of host immune and settle in the host to form persistent infections. In this study, we investigated the environmental adaptation mechanism of resistant Staphyloccus aureus (S. aureus) to host environment by Data-independent acquisition based quantitative proteomics and functional validation. The detection of growth curve and MIC indicated that ciprofloxacin-resistant S. aureus (Cip-R) showed survival advantage over sensitive strain. Cip-R also exhibited stronger adhesion and invasion ability than sensitive bacteria. Cip-R stimulation resulted in the production of stronger inflammatory factors of the host cells. Proteomics study combined with biochemical validations showed that Cip-R obtains adaptability to host via up-regulation of TCA cycle and down-regulation of ribosome metabolism and protein folding to maintain energy to support their survival. Thus, this study will help us to further explain the growth strategy of resistant bacteria to adapt to the host environment, and provide important information for the development of new antibacterial drugs.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_01:47:59.648.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liu jiajia
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-05 03:32:43	ID requested
⏵ 1	2022-05-31 01:48:01	announced

Liu J, Wei Q, Wang Z, Sun X, He QY, to the Host Environment. J Proteome Res, 21(6):1537-1547(2022) [pubmed]

submitter keyword: Staphylococcus aureus, Ciprofloxacin-resistant, Host environment, Proteomics

Qing-Yu He
contact affiliation	MOE Key Laboratory of Tumor Molecular Biology and Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
contact email	tqyhe@jnu.edu.cn
lab head
Liu jiajia
contact affiliation	Jinan univercity
contact email	2388269499@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029584

PRIDE project URI

• PRIDE
  1. PXD029584
     1. Label: PRIDE project
     2. Name: Adaptiven mechanism of antibiotic resistant Staphylococus aureus to host environment