PXD029574

PXD029574 is an original dataset announced via ProteomeXchange.

Title	Identification of METTL5 interaction partners
Description	METTL5 is an RNA methyltransferase whose stability was noted to be poor when expressed in both mammalian and bacterial expression systems. To determine if METTL5 has an endogenous stabilizing binding partner, FLAG-tagged METTL5 was expressed in Freestyle 293 cells and pulled down with anti-FLAG-M2 antibody-coupled magnetic beads. Bound proteins were fractionated by SDS-PAGE, and bands of interest were cut out for proteomics analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:43:03.132.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029574
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sigrid Nachtergaele
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	(R)-5-oxo-1,4-tetrahydrothiazine-3-carboxylic acid; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 10:17:43	ID requested
⏵ 1	2024-10-07 13:43:03	announced

10.6019/PXD029574;

10.1016/j.jbc.2022.101590;

Sepich-Poore C, Zheng Z, Schmitt E, Wen K, Zhang ZS, Cui XL, Dai Q, Zhu AC, Zhang L, Sanchez Castillo A, Tan H, Peng J, Zhuang X, He C, Nachtergaele S, -methyladenosine methyltransferase complex regulates mRNA translation via 18S rRNA methylation. J Biol Chem, 298(3):101590(2022) [pubmed]

submitter keyword: METTL5

Sigrid Nachtergaele
contact affiliation	Department of Molecular, Cellular, and Developmental Biology Yale University
contact email	sigrid.nachtergaele@yale.edu
lab head
Sigrid Nachtergaele
contact affiliation	Yale University
contact email	sigrid.nachtergaele@yale.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/01/PXD029574

PRIDE project URI

• PRIDE
  1. PXD029574
     1. Label: PRIDE project
     2. Name: Identification of METTL5 interaction partners