PXD029571

PXD029571 is an original dataset announced via ProteomeXchange.

Title	Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence
Description	This investigation reports a differential proteomic analysis of the secretome of six S. aureus strains belonging to two genotypes with opposite within-herd prevalence, GTB (high) and GTS (low), corresponding to sequence types (ST) 8 and 398, respectively. Total proteins released in the growth medium were subjected to high-resolution tandem mass spectrometry and differential analysis with Proteome Discoverer by label-free approach. Here, we reported both the characterization of secretome proteins and a panel of differential proteins specific of S. Aureus depending on high or low within-herd prevalence. GTB/ST8 (High) released more immunoglobulin-binding proteins, complement and antimicrobial peptide inhibitors, enterotoxins, and metabolic enzymes, while GTS/ST398 (Low) released more leukocidins, hemolysins, lipases, and peptidases. Furthermore, GTB/ST8 (High) released the von Willebrand factor protein, staphylokinase, and clumping factor B, while GTS/ST398 (Low) released the staphylococcal coagulase and clumping factor A. In conclusion, our results provide an in-depth characterization of secretome proteins of the three S. aureus GTB/ST8 and three GTS/ST398 strains with high and low within-herd prevalence. We describe the role of extracellular proteins in S. Aureus pathogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-02-14
AnnouncementXML	Submission_2022-02-14_01:42:10.329.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Salvatore Pisanu
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 10:17:28	ID requested
⏵ 1	2022-02-14 01:42:11	announced

Addis MF, Pisanu S, Monistero V, Gazzola A, Penati M, Filipe J, Di Mauro S, Cremonesi P, Castiglioni B, Moroni P, Pagnozzi D, Tola S, Piccinini R, strains with different within-herd intramammary infection prevalence. Virulence, 13(1):174-190(2022) [pubmed]

submitter keyword: Biological dataset, mammary gland, mastitis, Label-free proteomics, LC-MS/MS, bacterial virulence

Maria Filippa Addis
contact affiliation	Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Lodi, 26900, LO, Italy
contact email	filippa.addis@unimi.it
lab head
Salvatore Pisanu
contact affiliation	Porto Conte Ricerche, Alghero, Sardinia, Italy.
contact email	pisanu@portocontericerche.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029571
     1. Label: PRIDE project
     2. Name: Comparative secretome analysis of Staphylococcus aureus strains with different within-herd intramammary infection prevalence