PXD029563

PXD029563 is an original dataset announced via ProteomeXchange.

Title	IgG binds Escherichia coli serine protease EspP and protects mice from E. coli O157:H7 infection
Description	The effect of pooled immunoglobulins (IgG) on E. coli O157:H7 colonization and the course of disease in an EHEC mouse model was investigated showing an improved survival and decreased intestinal and renal pathology. Treatment was given after inoculation thereby corresponding to the clinical setting. In vitro studies identified E. coli serine protease EspP as the E. coli O157:H7 protein that IgG bound to, via the Fc fragment, in both murine and human IgG preparations, and blocked its enzymatic activity. EspP is a virulence factor previously shown to promote colonic cell injury and the uptake of Shiga toxin by intestinal cells. The results suggest that IgG in commercial preparations binds to EspP protecting the host from E. coli O157:H7 infection and could potentially be beneficial in patients.
HostingRepository	PRIDE
AnnounceDate	2022-02-08
AnnouncementXML	Submission_2022-02-08_07:42:17.331.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029563
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Charlotte Welinder
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 10:16:43	ID requested
⏵ 1	2022-02-08 07:42:17	announced

Dataset with its publication pending

submitter keyword: Escherichia coli O157:H7, Shiga toxin, EspP, immunoglobulin G, hemolytic uremic syndrome, mouse

Diana Ora Karpman
contact affiliation	Department of Pediatrics, Clinical Sciences Lund, Lund University, Lund, Sweden
contact email	diana.karpman@med.lu.se
lab head
Charlotte Welinder
contact affiliation	Dept. of Oncology, Clinical Sciences, Lund University, 221 85 Lund, Sweden
contact email	charlotte.welinder@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029563
     1. Label: PRIDE project
     2. Name: IgG binds Escherichia coli serine protease EspP and protects mice from E. coli O157:H7 infection