PXD029561

PXD029561 is an original dataset announced via ProteomeXchange.

Title	Monolaurin confers a protective effect against porcine epidemic diarrhea virus infection in piglets by regulating the interferon pathway
Description	Porcine epidemic diarrhea virus (PEDV) has reemerged as the main pathogen of piglets due to its high mutation feature. Monolaurin (ML) is a natural compound with a wide range of antibacterial and antiviral activities. However, the role of ML in PEDV infection is still unknown. This study aimed to evaluate the effect of ML on the growth performance, intestinal function, virus replication and cytokine response in piglets infected with PEDV, and to reveal the mechanism through proteomics analysis. Piglets were orally administrated with ML at a dose of 100 mg/kg·BW for 7 days before PEDV infection. Results showed that although there was no significant effect on the growth performance of piglets, ML administration alleviated the diarrhea caused by PEDV infection. ML administration promoted the recovery of intestinal villi, thereby improving intestinal function. Meanwhile, PEDV replication was significantly inhibited, and PEDV-induced expression of IL-6 and IL-8 were decreased with ML administration. Proteomics analyses showed that 38 proteins were differentially expressed between PEDV and ML+PEDV groups, and were significantly enriched in the interferon-related pathways. This suggests ML could promote the restoration of homeostasis by regulating the interferon pathway. Overall, the present study demonstrated ML could confer a protective effect against PEDV infection in piglets, and may be developed as a drug or feed additive to prevent and control PEDV disease.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_09:48:42.698.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qian Zhang
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 10:16:30	ID requested
⏵ 1	2022-02-16 09:48:44	announced

Zhang Q, Yi D, Ji C, Wu T, Wang M, Guo S, Wang L, Zhao D, Hou Y, Monolaurin Confers a Protective Effect Against Porcine Epidemic Diarrhea Virus Infection in Piglets by Regulating the Interferon Pathway. Front Immunol, 12():797476(2021) [pubmed]

submitter keyword: Monolaurin, porcine epidemic diarrhea virus, interferon, piglets, infection.

YONGQING HOU
contact affiliation	Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan, China
contact email	houyq@aliyun.com
lab head
Qian Zhang
contact affiliation	Wuhan polytechnic university
contact email	zhangqian870305@126.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029561
     1. Label: PRIDE project
     2. Name: Monolaurin confers a protective effect against porcine epidemic diarrhea virus infection in piglets by regulating the interferon pathway