PXD029558

PXD029558 is an original dataset announced via ProteomeXchange.

Title	Meta-proteomic analysis of two mammoth’s trunk for an indirect picture of their habitat and characterization of the alpha-1 type I collagen sequence
Description	The investigation of archaeological samples, which are of inestimable value, requires minimally destructive or non-destructive sampling techniques. One of the most promising and recent non-invasive techniques is known under the acronym EVA, an ethylene vinyl acetate film which avoids contamination and proteins damages. The present work reports the results of the metaproteomic analysis performed on the middle part of a trunk, and on the portion of a trunk tip tissue of two different woolly mammoths some 40000 years’ old. In particular, proteins were extracted by applying EVA (Ethylene-Vinyl Acetate) films to the surface of these tissues belonging to two Mammuthus primigenus specimens, discovered in two regions located in the Russian Far East, and then investigated via a shotgun MS-based approach. This approach allowed to obtain two interesting results: i) an indirect description of the habitat of these two mammoths, and ii) an improved sequence characterization of the alpha-1 type I collagen (col1a1), the major component of type I collagen. Sequence characterization of the col1a1 highlighted some differences between M. primigenius and other Proboscidea together with the identification of two potentially diagnostic amino acidic mutations that could be used to reliably distinguish the Mammuthus primigenius respect to the other two genera of elephantids (i.e. Elephas and Loxodonta), and the extinct American mastodon (i.e. Mammut americanum). The results were validated through the level of deamidation and other diagenetic chemical modifications of the sample peptides, which were used to discriminate the “original” endogenous peptides from contaminant ones.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_01:29:52.850.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029558
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vincenzo Cunsolo
SpeciesList	scientific name: Mammuthus primigenius; NCBI TaxID: 37349;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 09:09:27	ID requested
⏵ 1	2022-05-19 01:29:53	announced

Cucina A, Di Francesco A, Saletti R, Pittal, à MGG, Zilberstein G, Zilberstein S, Tikhonov A, Bublichenko AG, Righetti PG, Foti S, Cunsolo V, Meta-proteomic analysis of two mammoth's trunks by EVA technology and high-resolution mass spectrometry for an indirect picture of their habitat and the characterization of the collagen type I, alpha-1 and alpha-2 sequence. Amino Acids, 54(6):935-954(2022) [pubmed]

submitter keyword: Paleoproteomic, meta-proteomics, mammoth, high-resolution mass spectrometry, non-invasive technique, collagen

Salvatore Foti
contact affiliation	Department of Chemical Sciences, Organic Mass Spectrometry Laboratory, University of Catania, Via S. Sofia 64, 95123 Catania, Italy
contact email	sfoti@unict.it
lab head
Vincenzo Cunsolo
contact affiliation	University of Catania
contact email	vcunsolo@unict.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029558
     1. Label: PRIDE project
     2. Name: Meta-proteomic analysis of two mammoth’s trunk for an indirect picture of their habitat and characterization of the alpha-1 type I collagen sequence