PXD029558 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Meta-proteomic analysis of two mammoth’s trunk for an indirect picture of their habitat and characterization of the alpha-1 type I collagen sequence |
Description | The investigation of archaeological samples, which are of inestimable value, requires minimally destructive or non-destructive sampling techniques. One of the most promising and recent non-invasive techniques is known under the acronym EVA, an ethylene vinyl acetate film which avoids contamination and proteins damages. The present work reports the results of the metaproteomic analysis performed on the middle part of a trunk, and on the portion of a trunk tip tissue of two different woolly mammoths some 40000 years’ old. In particular, proteins were extracted by applying EVA (Ethylene-Vinyl Acetate) films to the surface of these tissues belonging to two Mammuthus primigenus specimens, discovered in two regions located in the Russian Far East, and then investigated via a shotgun MS-based approach. This approach allowed to obtain two interesting results: i) an indirect description of the habitat of these two mammoths, and ii) an improved sequence characterization of the alpha-1 type I collagen (col1a1), the major component of type I collagen. Sequence characterization of the col1a1 highlighted some differences between M. primigenius and other Proboscidea together with the identification of two potentially diagnostic amino acidic mutations that could be used to reliably distinguish the Mammuthus primigenius respect to the other two genera of elephantids (i.e. Elephas and Loxodonta), and the extinct American mastodon (i.e. Mammut americanum). The results were validated through the level of deamidation and other diagenetic chemical modifications of the sample peptides, which were used to discriminate the “original” endogenous peptides from contaminant ones. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_01:29:52.850.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029558 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Vincenzo Cunsolo |
SpeciesList | scientific name: Mammuthus primigenius; NCBI TaxID: 37349; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-04 09:09:27 | ID requested | |
⏵ 1 | 2022-05-19 01:29:53 | announced | |
Publication List
Cucina A, Di Francesco A, Saletti R, Pittal, à MGG, Zilberstein G, Zilberstein S, Tikhonov A, Bublichenko AG, Righetti PG, Foti S, Cunsolo V, Meta-proteomic analysis of two mammoth's trunks by EVA technology and high-resolution mass spectrometry for an indirect picture of their habitat and the characterization of the collagen type I, alpha-1 and alpha-2 sequence. Amino Acids, 54(6):935-954(2022) [pubmed] |
Keyword List
submitter keyword: Paleoproteomic, meta-proteomics, mammoth, high-resolution mass spectrometry, non-invasive technique, collagen |
Contact List
Salvatore Foti |
contact affiliation | Department of Chemical Sciences, Organic Mass Spectrometry Laboratory, University of Catania, Via S. Sofia 64, 95123 Catania, Italy |
contact email | sfoti@unict.it |
lab head | |
Vincenzo Cunsolo |
contact affiliation | University of Catania |
contact email | vcunsolo@unict.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029558
- Label: PRIDE project
- Name: Meta-proteomic analysis of two mammoth’s trunk for an indirect picture of their habitat and characterization of the alpha-1 type I collagen sequence