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PXD029558

PXD029558 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMeta-proteomic analysis of two mammoth’s trunk for an indirect picture of their habitat and characterization of the alpha-1 type I collagen sequence
DescriptionThe investigation of archaeological samples, which are of inestimable value, requires minimally destructive or non-destructive sampling techniques. One of the most promising and recent non-invasive techniques is known under the acronym EVA, an ethylene vinyl acetate film which avoids contamination and proteins damages. The present work reports the results of the metaproteomic analysis performed on the middle part of a trunk, and on the portion of a trunk tip tissue of two different woolly mammoths some 40000 years’ old. In particular, proteins were extracted by applying EVA (Ethylene-Vinyl Acetate) films to the surface of these tissues belonging to two Mammuthus primigenus specimens, discovered in two regions located in the Russian Far East, and then investigated via a shotgun MS-based approach. This approach allowed to obtain two interesting results: i) an indirect description of the habitat of these two mammoths, and ii) an improved sequence characterization of the alpha-1 type I collagen (col1a1), the major component of type I collagen. Sequence characterization of the col1a1 highlighted some differences between M. primigenius and other Proboscidea together with the identification of two potentially diagnostic amino acidic mutations that could be used to reliably distinguish the Mammuthus primigenius respect to the other two genera of elephantids (i.e. Elephas and Loxodonta), and the extinct American mastodon (i.e. Mammut americanum). The results were validated through the level of deamidation and other diagenetic chemical modifications of the sample peptides, which were used to discriminate the “original” endogenous peptides from contaminant ones.
HostingRepositoryPRIDE
AnnounceDate2022-05-19
AnnouncementXMLSubmission_2022-05-19_01:29:52.850.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD029558
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterVincenzo Cunsolo
SpeciesList scientific name: Mammuthus primigenius; NCBI TaxID: 37349;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-04 09:09:27ID requested
12022-05-19 01:29:53announced
Publication List
Cucina A, Di Francesco A, Saletti R, Pittal, à MGG, Zilberstein G, Zilberstein S, Tikhonov A, Bublichenko AG, Righetti PG, Foti S, Cunsolo V, Meta-proteomic analysis of two mammoth's trunks by EVA technology and high-resolution mass spectrometry for an indirect picture of their habitat and the characterization of the collagen type I, alpha-1 and alpha-2 sequence. Amino Acids, 54(6):935-954(2022) [pubmed]
Keyword List
submitter keyword: Paleoproteomic, meta-proteomics, mammoth, high-resolution mass spectrometry, non-invasive technique, collagen
Contact List
Salvatore Foti
contact affiliationDepartment of Chemical Sciences, Organic Mass Spectrometry Laboratory, University of Catania, Via S. Sofia 64, 95123 Catania, Italy
contact emailsfoti@unict.it
lab head
Vincenzo Cunsolo
contact affiliationUniversity of Catania
contact emailvcunsolo@unict.it
dataset submitter
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Dataset FTP location
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