PXD029556

PXD029556 is an original dataset announced via ProteomeXchange.

Title	Efficient TurboID-based proximate labeling method for identifying terminal sialic acid glycosylation in living cells
Description	TurboID, a mutant biotin ligase, based proximity labeling method is a new efficient technique for recognizing protein-protein interactions and has been successfully applied to living cells. Sialic acids are typically the terminal monosaccharides attached to many glycoproteins and play many important roles in many biological processes. However, the lack of enrichment methods for terminal sialic acid glycosylation in vivo hinders the identification and analysis of this glycosylation. Here, we introduced this technique to identify terminal sialic acid glycosylation in living cells. SpCBM, the carbohydrate-binding domain of sialidase from Streptococcus pneumoniae, was fused with TurboID and overexpressed in HeLa cells. After streptavidin-based purification and detection by mass spectrometry (MS), we identified 41 terminal sialic acid N-glycosylated sites and 3065 putative terminal sialic acid glycosylated proteins which many of them were located in cytoplasm and nucleus.
HostingRepository	iProX
AnnounceDate	2021-11-04
AnnouncementXML	Submission_2023-12-21_20:51:04.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wu Liu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-04 00:07:02	ID requested
⏵ 1	2023-12-21 20:51:05	announced

Liu W, Long Y, Bao Y, Li Y, Deng M, Yang X, Zhu H, Su Y, Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells. Acta Biochim Biophys Sin (Shanghai), 54(12):1841-1853(2022) [pubmed]

submitter keyword: TurboID, proximity lableing, glycosylation, sialic acid, SpCBM

Yanting Su
contact affiliation	Research Center of Basic Medical Sciences, School of Basic Medical Sciences, Hubei University of Science and Technology
contact email	2014202040134@whu.edu.cn
lab head
Wu Liu
contact affiliation	Hubei Key Laboratory of Diabetes and Angiopathy, Hubei University of Science and Technology
contact email	lw463684057@163.com
dataset submitter

iProX dataset URI