PXD029552 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | C. difficile strain 630 flagellin C modifications |
Description | In proteomics, the study of post-translational modifications often relies on some type of enrichment strategy. Here, we show that an approach that is commonly used in phosphoproteomics (Immobilised Metal Affinity Chromatography (Fe3+-IMAC)) also enriches for peptides with a unique post-translational modification, known as type A, in the human pathogen Clostridioides difficile. The type A modification consists of a monosaccharide (GlcNAc) that is linked to an N-methylated threonine through a phosphodiester bond. This structure has previously been described on flagellin C of several C. difficile strains and is important for bacterial motility. Using LC-MS/MS analyses of IMAC-captured tryptic peptides, we not only observed Type A modified C. difficile flagellin peptides but also a variety of truncated/modified Type A structures on these peptides. Using an elaborate set of mass spectrometry analyses, we demonstrate that one of these modifications consists of a Type A structure containing a phosphonate (2-aminoethylphosphonate, 2-AEP), a modification that is rarely observed and has hitherto not been described in C. difficile. |
HostingRepository | PRIDE |
AnnounceDate | 2022-04-04 |
AnnouncementXML | Submission_2022-04-03_18:23:47.246.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Yassene Mohammed |
SpeciesList | scientific name: Clostridioides difficile 630; NCBI TaxID: 272563; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-03 06:33:02 | ID requested | |
⏵ 1 | 2022-04-03 18:23:48 | announced | |
Publication List
Hensbergen PJ, de Ru AH, Friggen AH, Corver J, Smits WK, van Veelen PA, New insights into the type A glycan modification of Clostridioides difficile flagellar protein flagellin C by phosphoproteomics analysis. J Biol Chem, 298(3):101622(2022) [pubmed] |
Keyword List
submitter keyword: C. difficile, phosphoproteomics, flagellin |
Contact List
Paul J. Hensbergen |
contact affiliation | Center for Proteomics and Metabolomics Leiden University Medical Center Albinusdreef 2 2333 ZA Leiden The Netherlands |
contact email | P.J.Hensbergen@lumc.nl |
lab head | |
Yassene Mohammed |
contact affiliation | LUMC/UVIC |
contact email | y.mohammed@lumc.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029552
- Label: PRIDE project
- Name: C. difficile strain 630 flagellin C modifications