PXD029550

PXD029550 is an original dataset announced via ProteomeXchange.

Title	The long non-coding RNA DAGAR is regulated by m6A modification and both pathways are important for smooth muscle cell plasticity
Description	Vascular smooth muscle cells (SMCs) change between a contractile-differentiated and a proliferative-dedifferentiated phenotype in response to environmental cues. Long non-coding RNAs (lncRNAs) and N6-methyladenosine (m6A) modification regulate cell fate decision. Here we used primary human pulmonary artery SMCs (hPASMCs) and assessed the expression of lncRNAs during SMCs phenotypic modulation. A lncRNA, which we refer to as Differentiation And Growth Arrest-related lncRNA (DAGAR) was increased during differentiation and we demonstrate that it is required for this process. DAGAR was m6A-modified and regulated by the m6A reader YTHDF2 in SMCs and MRC5 cells. A marked downregulation of YTHDF1-3 proteins during both SMC differentiation and MRC5 quiescence was found, consistent with the increase of DAGAR. Remarkably, YTHDF2 immunoprecipitation followed by RNA deep sequencing (RIP-Seq) displayed an enrichment of key SMC-associated transcripts, including smooth muscle myosin heavy chain (MYH11) and members of the TGF, PDGF and VEGF pathways. Knockdown of YTHDF2 induced DAGAR and SMC marker gene expression. We conclude that the lncRNA DAGAR and YTHDF2 contribute to the regulation of SMC plasticity and differentiation programs.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:08:32.957.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029550
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Astrid Bruckmann
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidoethyl-L-cysteine; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-03 04:46:59	ID requested
⏵ 1	2025-05-06 09:08:33	announced

10.6019/PXD029550;

10.3390/ijms25179497;

de la Cruz-Thea B, Natali L, Ho-Xuan H, Bruckmann A, Coll-Bonfill N, Strieder N, Peinado VI, Meister G, Musri MM, ): Initial Characterization in Human Smooth Muscle and Fibroblast Cells. Int J Mol Sci, 25(17):(2024) [pubmed]

submitter keyword: smooth muscle cells,m6A modifcation, DAGAR

Astrid Bruckmann
contact affiliation	University Regensburg,Department of Biochemistry, geneticsand Microbiology, Chair of Biochemistry I
contact email	astrid.bruckmann@ur.de
lab head
Astrid Bruckmann
contact affiliation	University Regensburg
contact email	astrid.bruckmann@ur.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029550
     1. Label: PRIDE project
     2. Name: The long non-coding RNA DAGAR is regulated by m6A modification and both pathways are important for smooth muscle cell plasticity