Update information. Update publication information. During oogenesis, RNAs and proteins from maternal genome will be accumulated and they are the most important regulatory factors in early embryonic development. Parthenogenesis provides a unique source to investigate the influence of maternal genomes in early mammalian development and is proposed as an experimental tool to investigate embryo development which may solve many of the ethical concerns. Using label-free quantitative mass sepctrometry (MS), we systematically monitored protein expression profiles from six stages during pre-implantation development used by parthenogenesis model of mouse: pronucleus-, 2-cell, 4-cell, and 8-cell embryo, morula, and blastocyst. They are labeled as PA, PA-2, PA-4, PA-8, PAMO, PABL, respectively. For each stage, 6,000 embryos were used, and the experiment was performed in three biological replicates, and they had 2,048 proteins in common.