PXD029521

PXD029521 is an original dataset announced via ProteomeXchange.

Title	Differential proteomics of HeLa cells treated with PDS for 24 hours and untreated HeLa cells
Description	Pyridostatin (PDS) is a well-known G-quadruplex (G4) inducer and stabilizer, yet its target genes have remained unclear. Herein, combining mass spectrometry based proteomics strategy with bioinformatics analysis, we revealed that PDS significantly downregulated 22 proteins, of which the genes contain rich G4 potential sequences, in HeLa cancer cells, consequently upregulating 16 proteins remarkably. The PDS-regulated proteins appeared to work synergistically to activate cyclin and cell cycle regulation, and to restrain the inhibition of ARE-mediated mRNA decay pathway, suggesting that PDS itself is not a potential anticancer agent, at least towards HeLa cancer.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_10:51:05.241.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yinzhu Hou
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; monohydroxylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 07:24:37	ID requested
⏵ 1	2022-05-19 10:51:06	announced

Hou Y, Gan T, Fang T, Zhao Y, Luo Q, Liu X, Qi L, Zhang Y, Jia F, Han J, Li S, Wang S, Wang F, G-quadruplex inducer/stabilizer pyridostatin targets SUB1 to promote cytotoxicity of a transplatinum complex. Nucleic Acids Res, 50(6):3070-3082(2022) [pubmed]

submitter keyword: LC-MS/MS,HeLa

Fuyi Wang
contact affiliation	Institute of Chemistry, Chinese Academy of Sciences
contact email	fuyi.wang@iccas.ac.cn
lab head
Yinzhu Hou
contact affiliation	Institute of Chemistry, Chinese Academy of Sciences
contact email	yzhhou1995@iccas.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029521

PRIDE project URI

• PRIDE
  1. PXD029521
     1. Label: PRIDE project
     2. Name: Differential proteomics of HeLa cells treated with PDS for 24 hours and untreated HeLa cells