PXD029515

PXD029515 is an original dataset announced via ProteomeXchange.

Title	Differentiation of young and replicatively aged mouse C2C12 muscle cells
Description	Age-related impairments in myoblast differentiation may contribute to reductions in muscle function in older adults but the underlying proteostasis processes are not well understood. We investigated young (P6-10) and replicatively aged (P48-50) C2C12 myoblast cultures during early (0h-24h) and late (72h-96h) stages of differentiation using deuterium oxide (D2O) labelling and mass spectrometry. The absolute dynamic profiling technique for proteomics (Proteo-ADPT) was used to quantify the absolute rates of abundance change, synthesis and degradation of individual proteins. Proteo-ADPT encompassed 116 proteins and 74 proteins exhibited significantly (P<0.05, FDR <5 %) different changes in abundance between young and aged cells at early and later periods of differentiation. Young cells exhibited a steady pattern of growth, protein accretion and fusion, whereas aged cells failed to gain protein mass or undergo fusion during later differentiation. Maturation of the proteome was retarded in aged myoblasts at the onset of differentiation, but the proteome appeared to ‘catch up’ with the young cells during the early differentiation period. However, this ‘catch up’ process in aged cells was not accomplished by higher levels of protein synthesis. Instead, a lower level of protein degradation in aged cells was responsible for the elevated gains in protein abundance. Our novel data point to a loss of proteome quality as a precursor to the lack of fusion of aged myoblasts and highlights dysregulation of protein degradation, particularly of ribosomal and chaperone proteins, as a key mechanism that may contribute to age-related declines in the capacity of myoblasts to undergo differentiation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:01:46.483.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jatin Burniston
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 07:22:38	ID requested
1	2023-06-27 04:23:40	announced
⏵ 2	2023-11-14 09:01:55	announced	2023-11-14: Updated project metadata.

10.6019/PXD029515;

Brown AD, Stewart CE, Burniston JG, Degradation of ribosomal and chaperone proteins is attenuated during the differentiation of replicatively aged C2C12 myoblasts. J Cachexia Sarcopenia Muscle, 13(5):2562-2575(2022) [pubmed]

submitter keyword: Mouse, Deuterium Oxide, C2C12, Replicative ageing

Jatin Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
lab head
Jatin Burniston
contact affiliation	Liverpool John Moores University
contact email	j.burniston@ljmu.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD029515

PRIDE project URI

• PRIDE
  1. PXD029515
     1. Label: PRIDE project
     2. Name: Differentiation of young and replicatively aged mouse C2C12 muscle cells