PXD029512

PXD029512 is an original dataset announced via ProteomeXchange.

Title	nascent proteome analysis of HCT116 TP53 wild type and TP53 knockout cells treated with Neocarcinostatin (NCS) for 6h
Description	In order to investigate the potential role of TP53 in regulating translation, HCT116 wild type and TP53 knockout cells were analyzed using both RNA sequencing, Ribosome sequencing and nascent proteome analysis. The cells were treated with 0.2 µg/ml Neocarcinostatin (NCS) to induce DNA damage and activate TP53.
HostingRepository	PRIDE
AnnounceDate	2022-04-27
AnnouncementXML	Submission_2022-04-26_16:28:33.865.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029512
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Toman Bortecen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 06:53:39	ID requested
⏵ 1	2022-04-26 16:28:34	announced

Dataset with its publication pending

submitter keyword: nascent proteome, DNA damage, TP53, NCS, metabolic labeling

Jeroen Krijgsveld
contact affiliation	Division of Proteomics of Stem Cells and Cancer, German Cancer Research Center, Heidelberg, Germany
contact email	j.krijgsveld@dkfz.de
lab head
Toman Bortecen
contact affiliation	German Cancer Research Center (DKFZ)
contact email	toman@hotmail.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/04/PXD029512

PRIDE project URI

• PRIDE
  1. PXD029512
     1. Label: PRIDE project
     2. Name: nascent proteome analysis of HCT116 TP53 wild type and TP53 knockout cells treated with Neocarcinostatin (NCS) for 6h