PXD029509

PXD029509 is an original dataset announced via ProteomeXchange.

Title	PEP7 is a ligand for receptor kinase SIRK1 to regulate aquaporins and root growth
Description	Receptor kinases constitute the largest protein family in regulating various responses to external and internal biotic and abiotic signals. Functional characterization of this large protein family and particularly the identification of their ligands remains a major challenge in plant biology. Previously, we identified SIRK1 and QSK1 as a receptor / co-receptor pair involved in regulation of aquaporins in response to osmotic changes induced by sucrose. Here, we now identify a member of the Elicitor Peptide (PEP) family, namely PEP7, as a ligand to receptor kinase SIRK1. PEP7 was shown to bind to the extracellular domain of SIRK1 with a binding constant of 19 µM. PEP7 was secreted to the apoplasm specifically in response to sucrose. Formation of a signaling complex involving SIRK1, QSK1 as well as aquaporins as substrates was induced by sucrose or external PEP7 treatment. PEP7 induced aquaporin phosphorylation and water influx activity. The knock-out mutant of receptor SIRK1 was not responsive to external PEP7 treatment. Binding to receptor SIRK1 and induction of physiological responses was specific to PEP7, neither other members of the PEP-family (PEP6, PEP4), nor other small signaling peptides (CLEs, IDA, RALFs) induced SIRK1 kinase activity, aquaporin phosphorylation, or protoplast water influx activity.
HostingRepository	PRIDE
AnnounceDate	2025-05-06
AnnouncementXML	Submission_2025-05-06_09:06:26.308.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiahui Wang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 06:41:55	ID requested
⏵ 1	2025-05-06 09:06:27	announced

Xi L, Wu X, Wang J, Zhang Z, He M, Zeeshan Z, Stefan T, Schulze WX, Receptor Kinase Signaling of BRI1 and SIRK1 Is Tightly Balanced by Their Interactomes as Revealed From Domain-Swap Chimaera in AE-MS Approaches. Mol Cell Proteomics, 23(11):100857(2024) [pubmed]

10.1016/j.mcpro.2024.100857;

submitter keyword: receptor, aquaporins,ligand

Waltraud X Schulze
contact affiliation	Department of Plant Systems Biology
contact email	wschulze@uni-hohenheim.de
lab head
Jiahui Wang
contact affiliation	intitute of Biology
contact email	Jiahui.Wang@uni-hohenheim.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029509
     1. Label: PRIDE project
     2. Name: PEP7 is a ligand for receptor kinase SIRK1 to regulate aquaporins and root growth