PXD029505

PXD029505 is an original dataset announced via ProteomeXchange.

Title	Site-Link for identification of chromatinized proteome defined by site-specific histone acylations in living cells
Description	By coupling the mass spectrometry, we developed the Site-Link strategy based on the GCE method for analyzing the interacting proteome of histone lysine acylations in living cells. As K* could mimic the natural lysine to be recognized by natural lysine synthase, histones bearing the site-specifically incorporated K*acyl, should also be also integrated into nucleosomes and further compacted into chromatin similar as Kacyl with cell passaging. The subsequent 365-nm light irradiation could activate the diazirine and covalently capture potential effector proteins on native chromatin in living cells. The effector proteins could be identified after gel-based proteomics and MS/MS analysis of the crosslinked histone-effector complexes.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:39.226.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangfei Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 03:55:45	ID requested
1	2023-03-05 20:47:56	announced
⏵ 2	2023-11-14 08:33:40	announced	2023-11-14: Updated project metadata.

Qin F, Li B, Wang H, Ma S, Li J, Liu S, Kong L, Zheng H, Zhu R, Han Y, Yang M, Li K, Ji X, Chen PR, Linking chromatin acylation mark-defined proteome and genome in living cells. Cell, 186(5):1066-1085.e36(2023) [pubmed]

submitter keyword: HEK293T, histone acylation, site-specific, proteome, LC-MS/MS

Peng R. Chen
contact affiliation	Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Associate Editor, ACS Chemical Biology
contact email	pengchen@pku.edu.cn
lab head
Fangfei Qin
contact affiliation	Peking University
contact email	faqpku@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD029505

PRIDE project URI

• PRIDE
  1. PXD029505
     1. Label: PRIDE project
     2. Name: Site-Link for identification of chromatinized proteome defined by site-specific histone acylations in living cells