PXD029504

PXD029504 is an original dataset announced via ProteomeXchange.

Title	Identification of site-specific incorporation of Kacyl/K*acyl in full-length histones in E. coli and mammalian cells
Description	With the evolution of the PylRS system, we identified the PylRS variants for incorporation of all synthesized Kacyl and Kacyl* in the full-length H3 protein both in E. coli and mammalian cells, which covered the tail domain (eg. K23, K27, K56) as well as the globular domain that are difficult for chemical synthesis (eg. K64, K79, K122).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:31:19.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fangfei Qin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 03:55:33	ID requested
1	2023-03-05 20:37:23	announced
⏵ 2	2023-11-14 08:31:21	announced	2023-11-14: Updated project metadata.

Qin F, Li B, Wang H, Ma S, Li J, Liu S, Kong L, Zheng H, Zhu R, Han Y, Yang M, Li K, Ji X, Chen PR, Linking chromatin acylation mark-defined proteome and genome in living cells. Cell, 186(5):1066-1085.e36(2023) [pubmed]

submitter keyword: purified proteins, HEK293T,E. coli, LC-MS/MS

Peng R. Chen
contact affiliation	Professor and Chair of Chemical Biology, Peking-Tsinghua Center for Life Sciences Peking University, Beijing, China. Associate Editor, ACS Chemical Biology
contact email	pengchen@pku.edu.cn
lab head
Fangfei Qin
contact affiliation	Peking University
contact email	faqpku@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD029504

PRIDE project URI

• PRIDE
  1. PXD029504
     1. Label: PRIDE project
     2. Name: Identification of site-specific incorporation of Kacyl/K*acyl in full-length histones in E. coli and mammalian cells