PXD029494

PXD029494 is an original dataset announced via ProteomeXchange.

Title	Proteome profiling of Campylobacter jejuni 81-176 at 37°C and 42°C by label-free mass spectrometry
Description	Background: The main natural reservoir for Campylobacter jejuni is the intestinal tract of birds, especially in the context of human nutrition chickens and turkeys play the main role. There C. jejuni multiplies optimally at 42°C, the avian body temperature, while after infection of humans by peroral intake in the human intestinal tract only 37°C prevail. Methods: Proteome profiling by label-free mass spectrometry (SWATH-MS) was used to examine the cellular processes which enable C. jejuni strain 81-176 to adapt to 37°C in comparison to 42°C. In total, four states were compared with each other: incubation for 12 h at 37°C, for 24 h at 37°C, for 12 h at 42°C and 24 h at 42°C. Results: It was shown that the proteome profile changes not only according to the different incubation temperature but also with the length of the incubation period, i.e. significant differences in protein expression were already evident when comparing 12 h and 24 h. Altogether, the expression change of 957 proteins could be recorded. Out of these 171 proteins were considered to be significantly differentially expressed, that means these proteins showed at least a 1.5-fold change in either direction (i.e log2(FC)≥0.585 or log2(FC)≤-0.585) and an FDR-adjusted p-value less than 0.05. The significantly differentially expressed proteins could be arranged in 5 different clusters and several functional groups involving e.g. motility & chemotaxis, metabolism, stress response, rDNA translation and DNA replication.
HostingRepository	PRIDE
AnnounceDate	2024-06-16
AnnouncementXML	Submission_2024-06-15_23:28:24.287.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christof Lenz
SpeciesList	scientific name: Campylobacter jejuni; NCBI TaxID: 197;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 03:54:44	ID requested
⏵ 1	2024-06-15 23:28:25	announced

Dreyer A, Masanta WO, Lugert R, Bohne W, Gro, ß U, Leha A, Dakna M, Lenz C, Zautner AE, C by label-free mass spectrometry. BMC Microbiol, 24(1):191(2024) [pubmed]

10.1186/s12866-024-03348-8;

submitter keyword: mass spectrometry, temperature adaptation, data-independent acquisition, proteome,Campylobacter jejuni

Christof Lenz
contact affiliation	Core Facility Proteomics, Department of Clinical Medicine, University Medical Center Goettingen, Germany
contact email	christof.lenz@med.uni-goettingen.de
lab head
Christof Lenz
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	christof.lenz@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029494
     1. Label: PRIDE project
     2. Name: Proteome profiling of Campylobacter jejuni 81-176 at 37°C and 42°C by label-free mass spectrometry