PXD029490

PXD029490 is an original dataset announced via ProteomeXchange.

Title	Identification of novel protein binders of human MIRO2 in prostate cancer
Description	In previous studies, we showed that MIRO2 mRNA is up-regulated in primary cancer versus normal tissues, across multiple tumor types. MIRO2 had been previously studied in the context of non-tumorigenic cells, where it coordinates microtubule- and actin-based mitochondrial movement. However, we showed that cancer cells utilize MIRO2’s canonical function on mitochondrial trafficking exclusively under conditions of cellular stress that exacerbate mitochondrial dynamics. Thus, identifying MIRO2’s protein binding partners would shed light on the molecular function of MIRO2 in prostate cancer. To this end, we identified the proteins co-precipitating with FLAG-MIRO2 from PC3 cells. Network analysis of MIRO2 binding partners identified metabolism, cell cycle and cellular responses to extracellular stimuli amongst the top over-represented pathways.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_07:30:45.429.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029490
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Monika Dzieciatkowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-02 03:54:15	ID requested
⏵ 1	2021-11-25 07:30:45	announced

Dataset with its publication pending

submitter keyword: mitochondria, signaling, protein interaction, cancer

Cecilia Caino
contact affiliation	University of Colorado Anschutz Medical Campus,Department of Pharmacology
contact email	cecilia.caino@cuanschutz.edu
lab head
Monika Dzieciatkowska
contact affiliation	UC Denver
contact email	monika.dzieciatkowska@ucdenver.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD029490

PRIDE project URI

• PRIDE
  1. PXD029490
     1. Label: PRIDE project
     2. Name: Identification of novel protein binders of human MIRO2 in prostate cancer