PXD029460

PXD029460 is an original dataset announced via ProteomeXchange.

Title	Altered proteome in translationinitiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage
Description	The recognition of AUG start codon and selection of the open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the start codon selection adversely affectproteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein expression patterns in the translation initiation defectivehyper GTPaseeIF5G31R mutant cells. Consistently, we observed altered proteome involved in protein catabolism, nucleotide metabolism, fatty acid metabolism, glycolysis, and autophagy.The utilization of the upstream UUG codons by the eIF5G31R mutation caused downregulation of uridylate kinase expression and DNA damage. The eIF5G31R mutant cells showed lower Glutathione levels, high ROS activity, and sensitivity to H2O2.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_15:28:00.264.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pankaj Alone
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2021-11-01 05:00:00	ID requested
⏵ 1	2022-05-19 15:28:01	announced

Ram AK, Mallik M, Reddy RR, Suryawanshi AR, Alone PV, mutant causes oxidative stress and DNA damage. Sci Rep, 12(1):5033(2022) [pubmed]

submitter keyword: eIF5, Proteomics, Translation initiation, Sui¯ phenotype, iTRAQ

Pankaj V Alone
contact affiliation	NISER, Bhubaneswar, India
contact email	pankaj@niser.ac.in
lab head
Pankaj Alone
contact affiliation	National Institute of Science Education and Research, Bhubaneswar
contact email	pankaj@niser.ac.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029460
     1. Label: PRIDE project
     2. Name: Altered proteome in translationinitiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage