PXD029460 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Altered proteome in translationinitiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage |
Description | The recognition of AUG start codon and selection of the open reading frame (ORF) is fundamental to protein biosynthesis. Defect in the start codon selection adversely affectproteome and have a pleiotropic effect on cellular function. Using proteomic techniques, we identified differential protein expression patterns in the translation initiation defectivehyper GTPaseeIF5G31R mutant cells. Consistently, we observed altered proteome involved in protein catabolism, nucleotide metabolism, fatty acid metabolism, glycolysis, and autophagy.The utilization of the upstream UUG codons by the eIF5G31R mutation caused downregulation of uridylate kinase expression and DNA damage. The eIF5G31R mutant cells showed lower Glutathione levels, high ROS activity, and sensitivity to H2O2. |
HostingRepository | PRIDE |
AnnounceDate | 2022-05-19 |
AnnouncementXML | Submission_2022-05-19_15:28:00.264.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pankaj Alone |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | No PTMs are included in the dataset |
Instrument | 4800 Proteomics Analyzer |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-01 05:00:00 | ID requested | |
⏵ 1 | 2022-05-19 15:28:01 | announced | |
Publication List
Ram AK, Mallik M, Reddy RR, Suryawanshi AR, Alone PV, mutant causes oxidative stress and DNA damage. Sci Rep, 12(1):5033(2022) [pubmed] |
Keyword List
submitter keyword: eIF5, Proteomics, Translation initiation, Sui¯ phenotype, iTRAQ |
Contact List
Pankaj V Alone |
contact affiliation | NISER, Bhubaneswar, India |
contact email | pankaj@niser.ac.in |
lab head | |
Pankaj Alone |
contact affiliation | National Institute of Science Education and Research, Bhubaneswar |
contact email | pankaj@niser.ac.in |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029460
- Label: PRIDE project
- Name: Altered proteome in translationinitiation fidelity defective eIF5G31R mutant causes oxidative stress and DNA damage