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PXD029459

PXD029459 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative analysis of PRE DNA-binding factors interactomes: purification of Combgap, Zeste, Psq and Adf1 associated proteins
DescriptionThe Polycomb group (PcG) and Trithorax group (TrxG) proteins are key epigenetic regulators controlling silenced and active states of genes in multicellular organisms, respectively. In Drosophila PcG/TrxG proteins are recruited to chromatin via binding to specialized DNA-elements termed Polycomb Response Elements (PREs). While precise mechanisms of PcG/TrxG proteins recruitment remains unknown, the important role is suggested to belong to sequence specific DNA-binding factors. Here, using affinity purification coupled with high throughput mass spectrometry (IP/MS), we isolated factors associated with Combgap, Psq, Zeste and Adf1 PRE DNA-binding Drosophila proteins. As a control we used unspecific IgG. For affinity purification the nuclear extract from Drosophila S2 cells and polyclonal antibodies against Combgap, Psq, Zeste and Adf1 were used.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:40:52.553.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD029459
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRustam Ziganshin
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-11-01 04:59:49ID requested
12024-05-22 01:12:49announced
22024-10-22 06:40:54announced2024-10-22: Updated project metadata.
Publication List
10.1007/s00018-022-04383-2;
10.6019/PXD029459;
Chetverina D, Vorobyeva NE, Mazina MY, Fab LV, Lomaev D, Golovnina A, Mogila V, Georgiev P, Ziganshin RH, Erokhin M, Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins. Cell Mol Life Sci, 79(7):353(2022) [pubmed]
Keyword List
submitter keyword: interactomes, DNA-binding factors,Drosophila melanogaster, LC-MS/MS
Contact List
Maksim Erokhin
contact affiliationInstitute of Gene Biology, Russian Academy of Sciences, Group of Chromatin Biology
contact emailyermaxbio@yandex.ru
lab head
Rustam Ziganshin
contact affiliationShemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences
contact emailrustam.ziganshin@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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