PXD029459 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative analysis of PRE DNA-binding factors interactomes: purification of Combgap, Zeste, Psq and Adf1 associated proteins |
Description | The Polycomb group (PcG) and Trithorax group (TrxG) proteins are key epigenetic regulators controlling silenced and active states of genes in multicellular organisms, respectively. In Drosophila PcG/TrxG proteins are recruited to chromatin via binding to specialized DNA-elements termed Polycomb Response Elements (PREs). While precise mechanisms of PcG/TrxG proteins recruitment remains unknown, the important role is suggested to belong to sequence specific DNA-binding factors. Here, using affinity purification coupled with high throughput mass spectrometry (IP/MS), we isolated factors associated with Combgap, Psq, Zeste and Adf1 PRE DNA-binding Drosophila proteins. As a control we used unspecific IgG. For affinity purification the nuclear extract from Drosophila S2 cells and polyclonal antibodies against Combgap, Psq, Zeste and Adf1 were used. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:40:52.553.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD029459 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Rustam Ziganshin |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-11-01 04:59:49 | ID requested | |
1 | 2024-05-22 01:12:49 | announced | |
⏵ 2 | 2024-10-22 06:40:54 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1007/s00018-022-04383-2; |
10.6019/PXD029459; |
Chetverina D, Vorobyeva NE, Mazina MY, Fab LV, Lomaev D, Golovnina A, Mogila V, Georgiev P, Ziganshin RH, Erokhin M, Comparative interactome analysis of the PRE DNA-binding factors: purification of the Combgap-, Zeste-, Psq-, and Adf1-associated proteins. Cell Mol Life Sci, 79(7):353(2022) [pubmed] |
Keyword List
submitter keyword: interactomes, DNA-binding factors,Drosophila melanogaster, LC-MS/MS |
Contact List
Maksim Erokhin |
contact affiliation | Institute of Gene Biology, Russian Academy of Sciences, Group of Chromatin Biology |
contact email | yermaxbio@yandex.ru |
lab head | |
Rustam Ziganshin |
contact affiliation | Shemyakin-Ovchinnikov Institute of bioorganic chemistry Russian Academy of Sciences |
contact email | rustam.ziganshin@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD029459
- Label: PRIDE project
- Name: Comparative analysis of PRE DNA-binding factors interactomes: purification of Combgap, Zeste, Psq and Adf1 associated proteins