PXD029444

PXD029444 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Development of sequential sample processing protocol on a single anion-exchange solid-phase extraction disc for bottom-up/cross-linking mass spectrometry
Description	In this project, we developed a rapid enzymatic digestion protocol within an anion exchange StageTip named AnExSP (anion-exchange disc-assisted sequential sample preparation) and, using data-independent acquisition, analyzed its digestion performance of human cellular protein extracts (HCPE) using trypsin and trypsin/Lys-C. The outstanding effectiveness and simplicity of AnExSP in sample preparation for gel-based cross-linking mass spectrometry was also verified.
HostingRepository	jPOST
AnnounceDate	2022-04-20
AnnouncementXML	Submission_2022-09-18_03:19:24.257.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yusuke Kawashima
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; unknown modification
Instrument	Q Exactive HF-X; Orbitrap Exploris 480

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-29 16:34:04	ID requested
1	2022-04-20 00:54:51	announced
2	2022-04-20 00:56:08	announced	2022-04-20: Updated PubMed.
⏵ 3	2022-09-18 03:19:25	announced	2022-09-18: Updated FTP location.

Publication List

Takemori A, Kawashima Y, Takemori N, simplified sample processing on anion-exchange solid-phase extraction spin column. Chem Commun (Camb), 58(6):775-778(2022) [pubmed]

Takemori A, Kawashima Y, Takemori N, simplified sample processing on anion-exchange solid-phase extraction spin column. Chem Commun (Camb), 58(6):775-778(2022) [pubmed]

Keyword List

submitter keyword: AnExSP, PEPPI, in-gel enzymatic digestion, XL-MS, DIA-MS

submitter keyword: AnExSP, PEPPI, in-gel enzymatic digestion, XL-MS, DIA-MS

Contact List

Nobuaki Takemori
lab head
Yusuke Kawashima
contact affiliation	Kazusa DNA Research Institute
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001370/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001370/