PXD029439

PXD029439 is an original dataset announced via ProteomeXchange.

Title	Antigen discovery in circulating extracellular vesicles from Plasmodium vivax patients
Description	Plasmodium vivax is the most widely distributed human malaria parasite with 7 million annual clinical cases and 2.5 billion people living under risk of infection. Presently, there is an urgent need to discover new antigens for vaccination as only two vaccine candidates, both based on the Duffy Binding protein, are currently in clinical trials. Extracellular vesicles (EVs) are small membrane-bound vesicles involved in intercellular communication and initially described in reticulocytes, the host cell of P. vivax, as a selective disposal mechanism of the transferrin receptor (CD71) in the maturation of reticulocytes to erythrocytes. We have recently reported the proteomics identification of P. vivax proteins associated to circulating EVs in P. vivax patients using size exclusion chromatography followed by mass spectrometry. Parasite proteins, however, were detected in two out of ten patients and only three of them with more than one unique peptide (UP). To increase the signal, we have implemented the Direct Immuno-affinity Capture (DIC) technique to enrich in EVs derived from CD71-expressing cells. Remarkably, we identified parasite proteins in all patients totaling 48 proteins (24 identified with ≥2 UP) and including several previously identified P. vivax vaccine candidate antigens (MSP1, MSP3, MSP7, MSP9, Serine-repeat antigen 1, and HSP70) as well as several membrane, cytosolic and exported proteins. Notably, a member of the Plasmodium helical interspersed sub-telomeric (PHIST-c) family, previously shown to be a main component of the caveola vesicle complexes, was robustly detected in five out six analyzed patients. Humoral immune response analysis by luminex confirmed its antigenicity. Collectively, we showed that enrichment of EVs by CD71-DIC from plasma, allows a robust identification of P. vivax proteins. This study represents a major advance in identifying new antigens for vaccination against this human malaria parasite.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:31:05.006.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eva Borràs
SpeciesList	scientific name: Plasmodium vivax; NCBI TaxID: 5855;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-29 06:52:26	ID requested
⏵ 1	2022-02-22 04:31:05	announced

Aparici-Herraiz I, Gualdr, ó, n-L, ó, pez M, Castro-Cavad, í, a CJ, Carmona-Fonseca J, Yasnot MF, Fernandez-Becerra C, Del Portillo HA, Patients. Front Cell Infect Microbiol, 11():811390(2021) [pubmed]

submitter keyword: Plasmodium Vivax. extracellular vesicles, mass spectrometry

Eduard Sabido
contact affiliation	CRG / UPF
contact email	eduard.sabido@crg.eu
lab head
Eva Borràs
contact affiliation	UPF
contact email	eva.borras@upf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029439

PRIDE project URI

• PRIDE
  1. PXD029439
     1. Label: PRIDE project
     2. Name: Antigen discovery in circulating extracellular vesicles from Plasmodium vivax patients