PXD029419

PXD029419 is an original dataset announced via ProteomeXchange.

Title	Proteomics of the periodontal pathogen Prevotella intermedia secretome in biofilm and planktonic lifestyles
Description	Protein secretion into extracellular space is an important virulence mechanism both among Gram negative and Gram-positive bacteria. Prevotella intermedia, an important species associated with periodontitis, is known to be resistant to several antibiotics. Since P. intermedia is a part of normal oral microbiota, its complete elimination is not possible. Despite the remarkable clinical significance P. intermedia has, little is known about the molecular basis for its virulence. The aim of this study was to characterize the secretome of P. intermedia in biofilm and planktonic life mode. Proteins in the secretome preparations were identified by nanoLC-ESI-MS/MS. The biofilm secretome showed 109 proteins while the planktonic secretome showed 136 proteins. The biofilm and the planktonic secretomes contained 17 and 33 signal-peptide bearing proteins, 13 and 18 lipoproteins, respectively. Proteins with predicted virulence potential were 39 in biofilm and 44 in planktonic secretomes, respectively. Gene ontology analysis revealed that the biofilm secretome displayed a markedly higher percent proteins compared to planktonic secretome in terms of cellular amino acid metabolic process, nitrogen compound metabolic process, protein binding and methyltransferase and kinase activities. In conclusion, this study revealed differences in the protein profiles of P. intermedia biofilm and planktonic secretomes. This may set a basis for asking further questions into molecular mechanisms how this species exerts its virulence potential in the oral cavity.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_13:59:45.368.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maribasappa Karched
SpeciesList	scientific name: Prevotella intermedia ATCC 25611 = DSM 20706; NCBI TaxID: 1122984;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-29 04:25:11	ID requested
⏵ 1	2022-05-19 13:59:46	announced

Karched M, Bhardwaj RG, Qudeimat M, Al-Khabbaz A, Ellepola A, Proteomic analysis of the periodontal pathogen Prevotella intermedia secretomes in biofilm and planktonic lifestyles. Sci Rep, 12(1):5636(2022) [pubmed]

submitter keyword: Prevotella, secretome, proteomics, biofilm, planktonic, virulence

Maribasappa Karched
contact affiliation	Oral Microbiology Research Laboratory Faculty of Dentistry Kuwait University
contact email	maribasappa.karched@gmail.com
lab head
Maribasappa Karched
contact affiliation	Faculty of Dentistry Kuwait University
contact email	maribasappa.karched@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029419

PRIDE project URI

• PRIDE
  1. PXD029419
     1. Label: PRIDE project
     2. Name: Proteomics of the periodontal pathogen Prevotella intermedia secretome in biofilm and planktonic lifestyles