PXD029408

PXD029408 is an original dataset announced via ProteomeXchange.

Title	mTOR inhibition via Rapamycin treatment partially reverts the deficit in energy metabolism caused by FH loss in RPE cells
Description	To obtain insight into the molecular mechanism of FH intracellular function in hTERT-RPE1 cells, we analyzed the interactome of FH via its immunoprecipitation followed by MS-based analysis. We found that FH interacts with essential components of the ubiquitin-proteasomal pathway (UPS) as well as with RB1/E2F signalling in a complement-pathway independent manner. Moreover, we found, that FH silencing affects mRNA levels of the E3 Ubiquitin-Protein Ligase Parkin and PTEN induced putative kinase (Pink1), both of them are associated with UPS. As inhibition of mTORC1 has been previoulsy shown to result in increased overall protein degradation via UPS and as FH mRNA and protein levels were shown to be affected by inhibition of UPS, our data stress a potential regulatory link between endogenous FH activity and UPS.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_12:43:35.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mohamed Ali Jarboui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-28 06:42:45	ID requested
⏵ 1	2022-02-16 12:43:36	announced

Merle DA, Provenzano F, Jarboui MA, Kilger E, Clark SJ, Deleidi M, Armento A, Ueffing M, mTOR Inhibition via Rapamycin Treatment Partially Reverts the Deficit in Energy Metabolism Caused by FH Loss in RPE Cells. Antioxidants (Basel), 10(12):(2021) [pubmed]

submitter keyword: AMD, Complement Factor H, Interactome, AP-Proteomics, mTOR

Angela Armento
contact affiliation	Institute for Ophthalmic Research Eberhard-Karls University of Tuebingen Elfriede-Aulhorn-Str. 7 D-72076 Tuebingen. Germany.
contact email	angela.armento@uni-tuebingen.de
lab head
Mohamed Ali Jarboui
contact affiliation	Medical Bioanalytics, University Clinic Tuebingen
contact email	mohamed-ali.jarboui@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029408

PRIDE project URI

• PRIDE
  1. PXD029408
     1. Label: PRIDE project
     2. Name: mTOR inhibition via Rapamycin treatment partially reverts the deficit in energy metabolism caused by FH loss in RPE cells