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PXD029393

PXD029393 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleChaperone-mediated autophagy maintains glioma stem cell activity controlling different proteomic and transcriptomic pathways (U251)
DescriptionChaperone-mediated autophagy (CMA) is a homeostatic process essential for the lysosomal degradation of individual proteins. CMA activity directly depends on the levels of LAMP2A, critical receptor at the lysosomal membrane, to which CMA substrate proteins are bound. In glioblastoma (GBM), the most common and aggressive brain cancer in the adulthood, high levels of LAMP2A have been linked to TMZ resistance and tumor-associated pericytes. However, the implication of LAMP2A, and hence CMA, in glioblastoma stem cells (GSCs) remains unknown. In this work, we show that LAMP2A expression is enriched in patient-derived GSC population and its knock-down diminishes GSCs tumorigenic activities. Proteomic and transcriptomic analysis of LAMP2A knocked-down GSCs revealed reduced extracellular matrix (ECM) interaction effectors in both analyses. Moreover, immune system and mitochondrial metabolism related pathways were remarkably deregulated at proteome level, whereas PI3K-AKT and p53 pathways were altered in RNAseq study. Moreover, clinical samples of GBM tissue presented overexpression of LAMP2 and its high levels correlated with advanced glioma grade and poor overall survival. In conclusion, we identified a novel role of CMA directly regulating GSCs activity via multiple pathways at proteome and transcriptome level.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:54:13.347.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMikel Azkargorta
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-10-28 01:54:19ID requested
12022-08-02 08:24:16announced
22023-11-14 08:54:13announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: glioblastoma,Chaperone-mediated autophagy, LAMP2A, label free
Contact List
Félix Elortza
contact affiliationProteomics Service CIC bioGUNE Bizkaia Tech. Park Build. 800 Derio 48160 Spain
contact emailfelortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliationProteomics Platform CIC bioGUNE
contact emailmazkargorta@cicbiogune.es
dataset submitter
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Dataset FTP location
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