PXD029379

PXD029379 is an original dataset announced via ProteomeXchange.

Title	Label-free quantification (LFQ) analysis on skeletal muscle stem cells and reprogrammed skeletal muscle stem and progenitor cells
Description	Direct lineage reprogramming provides a unique system to study cell fate transitions and unearth molecular mechanisms that safeguard cellular identity. We previously reported on direct conversion of mouse fibroblasts into induced myogenic progenitor cells (iMPCs) by transient MyoD overexpression in concert with small molecules treatment. Here we employed integrative multi-omic approaches to delineate the molecular landscape of fibroblast reprogramming into iMPCs in comparison to transdifferentiation into myogenic cells solely by MyoD overexpression. Utilizing bulk RNA-sequencing and mass spectrometry, we uncovered molecular regulators and pathways that endow a myogenic stem cell identity on fibroblasts only in the presence of small molecule treatment. In addition, we demonstrate that Pax7+ cells in iMPCs share molecular attributes with myoblasts, however in addition express unique genes, proteins and pathways that are indicative of a more activated satellite cell-like state in vitro. Collectively, this study charts a molecular blueprint for reprogramming fibroblasts into muscle stem and progenitor cells and further establishes the fidelity of stable iMPC cultures in capturing skeletal muscle regeneration in vitro for disease modeling and basic research applications.
HostingRepository	PRIDE
AnnounceDate	2022-05-19
AnnouncementXML	Submission_2022-05-19_13:39:22.343.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Adhideb Ghosh
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-27 08:13:39	ID requested
⏵ 1	2022-05-19 13:39:23	announced

Kim I, Ghosh A, Bundschuh N, Hinte L, Petrosyan E, von Meyenn F, Bar-Nur O, Integrative molecular roadmap for direct conversion of fibroblasts into myocytes and myogenic progenitor cells. Sci Adv, 8(14):eabj4928(2022) [pubmed]

submitter keyword: Reprogramming, Skeletal muscle, Satellite cells, LFQ

Prof. Dr. Ori Bar-Nur
contact affiliation	Laboratory of Regenerative and Movement Biology, Dept. of Health Sciences and Technology ETH Zurich, Schorenstrasse 16, 8603 Schwerzenbach, Switzerland
contact email	ori.bar-nur@hest.ethz.ch
lab head
Adhideb Ghosh
contact affiliation	ETH Zurich
contact email	adhideb.ghosh@fgcz.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029379

PRIDE project URI

• PRIDE
  1. PXD029379
     1. Label: PRIDE project
     2. Name: Label-free quantification (LFQ) analysis on skeletal muscle stem cells and reprogrammed skeletal muscle stem and progenitor cells