PXD029358
PXD029358 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Function of two GSA isoforms in Arabidopsis thaliana |
| Description | As proof of interdependent interaction between GSAT, GluTR, and GBP, a crosslinking mass spectrometry experiment was conducted with bilateral and trilateral combinations of the three respective recombinant proteins. Apart from the known GBP – GluTR interaction, combined assays for mutual interaction of GSAT either with GluTR or GBP were carried out and ultimately a crosslinking experiment was performed including all three proteins. The proteins of these assays were subsequently cleaved by trypsin and analysed by mass spectrometry to identify the crosslinked peptides of the three proteins in all possible of dual and triple combinations. This approach enabled the detection of the interrelation between all three proteins. |
| HostingRepository | jPOST |
| AnnounceDate | 2022-11-16 |
| AnnouncementXML | Submission_2023-02-06_23:54:42.329.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jürgen Eirich |
| SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
| ModificationList | S-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide; unknown modification |
| Instrument | Q Exactive |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2021-10-26 02:08:22 | ID requested | |
| 1 | 2022-11-15 07:00:03 | announced | |
| ⏵ 2 | 2023-02-06 23:54:43 | announced | 2023-02-07: Updated PubMed. |
Publication List
| Sinha N, Eirich J, Finkemeier I, Grimm B, Glutamate 1-semialdehyde aminotransferase is connected to GluTR by GluTR-binding protein and contributes to the rate-limiting step of 5-aminolevulinic acid synthesis. Plant Cell, 34(11):4623-4640(2022) [pubmed] |
Keyword List
| submitter keyword: cross link, PPI |
Contact List
| Bernhard Grimm | |
|---|---|
| lab head | |
| Jürgen Eirich | |
| contact affiliation | University of Münster - WWU - Institute for Biology and Biotechnology of Plants |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001362/ |
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