PXD029356

PXD029356 is an original dataset announced via ProteomeXchange.

Title	Label-free and targeted mass tandem (TMT-labelled) global proteomic analyses on mucus from the stomach, small and large intestine of a domestic and laboratory dogs
Description	Gastrointestinal (GI) mucus is continuously secreted and lines the entire length of the GI tract. Essential for health, it keeps the noxious luminal content away from the epithelium and propels forward the digesta. The aim of our study was to characterize the composition and structures of mucus throughout the various GI segments in dog. Mucus from the stomach, small intestine (duodenum, jejunum, ileum), and large intestine (cecum, proximal and distal colon) was collected from 5 dogs. pH and water content of GI mucus and digesta were analyzed. Composition of all GI-tract segments from a domestic and a laboratory dog was determined by label-free global proteomics. A colonic-focussed composition analysis with TMT-labelled proteomics was used on jenunal and proximal and distal colonic mucus samples from 3 laboratory and 1 domestic dog. Finally, the composition of jejunal and colonic mucus samples of 3 laboratory and 1 domestic dog was evaluated with lipidomics and metabolomics. Structural properties were investigated using cryoSEM and rheology. The proteome was similar across the different GI segments. The highest abundant secreted gel-forming mucin in the gastric mucus was mucin 5AC, whether mucin 2 had highest abundance in the intestinal mucus. Lipid and metabolite abundance was generally higher in the jejunal mucus than the colonic mucus. In conclusion, the mucus is a highly viscous and hydrated material. The proteins, lipids and metabolites were similar throughout the GI tract, although abundances depended on location. These data provide an important baseline for future studies on human and canine intestinal diseases and the dog model in drug absorption.
HostingRepository	PRIDE
AnnounceDate	2022-03-07
AnnouncementXML	Submission_2022-03-07_07:21:22.735.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaofang Cao
SpeciesList	scientific name: Canis lupus familiaris; NCBI TaxID: 9615;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-26 01:24:35	ID requested
⏵ 1	2022-03-07 07:21:23	announced

Dubbelboer IR, Barmpatsalou V, Rodler A, Karlsson E, Nunes SF, Holmberg J, H, ä, ggstr, ö, m J, Bergstr, ö, m CAS, Gastrointestinal mucus in dog: Physiological characteristics, composition, and structural properties. Eur J Pharm Biopharm, 173():92-102(2022) [pubmed]

submitter keyword: dog, canine, gastrointestinal, mucus, healthy, composition

Janne Lehtiö
contact affiliation	Dept. of Oncology-Pathology, Karolinska Institutet, SciLifeLab, Stockholm, Sweden
contact email	Janne.Lehtio@ki.se
lab head
Xiaofang Cao
contact affiliation	Karolinska Institutet
contact email	xiaofang.cao@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029356

PRIDE project URI

• PRIDE
  1. PXD029356
     1. Label: PRIDE project
     2. Name: Label-free and targeted mass tandem (TMT-labelled) global proteomic analyses on mucus from the stomach, small and large intestine of a domestic and laboratory dogs