PXD029354

PXD029354 is an original dataset announced via ProteomeXchange.

Title	Human cell lines pulldown interactions CL-MSMS
Description	Using a combination of GFP-trap affinity purification, native gel band identification and APEX2-mediated BioID pulse-chase analysis, we investigate an import pathway and folding mechanism for histones H3 and H4. We investigated proteins associating with H3 and H4 bearing mutations along alpha-helix 2 using affinity-capture followed by on-beads trypsin digestion and label-free mass spectrometry. In addition, we investigated the composition subcomplexes of affinity-purified histone chaperone NASP separated by native-PAGE, followed by in-gel trypsin digestion and label-free mass spectrometry. Finally, we investigated the proximity in vivo to histone H3 construct under a pulse-chase system (RAPID-release) using two different time points for APEX2-based biotin-phenol biotinylation, 0 minutes and 120 minutes. Samples were quenched and streptavidin-purified, followed by on-beads digestion and label-free mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2022-09-15
AnnouncementXML	Submission_2022-09-15_13:33:11.699.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029354
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-26 01:24:17	ID requested
⏵ 1	2022-09-15 13:35:19	announced

Pardal AJ, Bowman AJ, A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3. Elife, 11():(2022) [pubmed]

submitter keyword: Human, HEK293-F, HeLa FRT, BioID, histone chaperone, LC-MSMS, Warwick_RTP

Andrew Bowman
contact affiliation	Warwick Medical School, University of Warwick, Coventry, CV4 7AL, United Kingdom
contact email	a.bowman.1@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029354
     1. Label: PRIDE project
     2. Name: Human cell lines pulldown interactions CL-MSMS