PXD029330

PXD029330 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteomics analysis on cottonseed meal protein isolate extract-ed using improved gossypol removal method
Description	Cottonseed meal (CSM) contains a high amount of underutilised proteins due to the presence of gossypol, a toxic compound unsuitable for human consumption. We designed a protein extract process to generate CSM protein isolate (CSMPI) with ultra-low gossypol content to unlock their potential. This process utilised alkaline-based protein extraction with gossypol removal through a multistep acidified organic solvent treatment. Overall, our method successfully yielded 22% of CSMPI from CSM and achieved a protein purity of 92.6%. The method has reduced the free and total gossypol content of the post-treated CSMPI to 4.8ppm and 147.2ppm, respectively, which is much lower than the limit set by US FDA. Proteomics analysis indicated that the gossypol re-moval treatment increased protein identification and enhanced the nutritional quality of the CSMPI due to increased branched-chain amino acids (BCAAs) content after treatment. Overall, our results help to navigate and direct the application of CSMPI towards nutritive human food application.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:17:30.244.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Soon Hong Kwan
SpeciesList	scientific name: Gossypium hirsutum; NCBI TaxID: 3635;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 6600

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-25 02:42:55	ID requested
1	2022-10-13 14:30:52	announced
⏵ 2	2023-11-14 07:17:31	announced	2023-11-14: Updated project metadata.

Publication List

Tan CF, Kwan SH, Lee CS, Soh YNA, Ho YS, Bi X, Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective. Int J Mol Sci, 23(17):(2022) [pubmed]

Tan CF, Kwan SH, Lee CS, Soh YNA, Ho YS, Bi X, Cottonseed Meal Protein Isolate as a New Source of Alternative Proteins: A Proteomics Perspective. Int J Mol Sci, 23(17):(2022) [pubmed]

Keyword List

submitter keyword: cottonseed meal
gossypol
proteomics
acidified organic solvent-based gossypol removal
alka-line-based protein solubilisation
allergen

submitter keyword: cottonseed meal

gossypol

proteomics

acidified organic solvent-based gossypol removal

alka-line-based protein solubilisation

allergen

Contact List

Bi Xuezhi
contact affiliation	Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 138668, Singapore.
contact email	bi_xuezhi@bti.a-star.edu.sg
lab head
Soon Hong Kwan
contact affiliation	Bioprocessing Technology Institute (BTI), Agency for Science, Technology and Research (A*STAR), 138668, Singapore
contact email	sebastian_kwan@bti.a-star.edu.sg
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD029330

PRIDE project URI

Repository Record List

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