PXD029307

PXD029307 is an original dataset announced via ProteomeXchange.

Title	Novel tissue-specific markers revealed by in-depth proteome analysis of human myotendinous junction
Description	Force is transmitted between skeletal muscle and tendon via a highly specialized tissue interface: the myotendinous junction (MTJ). Despite its crucial role in mechano-transduction and involvement in muscle strain injury, knowledge about the molecular composition of the MTJ is incomplete. We performed LCMS-based proteomic analysis of human MTJ by progressive removal of the muscle component in the human semitendinosus muscle-tendon complex. Patellar tendon, which does not contains muscle tissue, was used as control. We quantified >3000 proteins across all samples and 112 proteins were significantly increased in MTJ. Of these, 24 proteins have previously been identified as MTJ proteins, including the MTJ-marker collagen XXII. Moreover, an overrepresentation of proteins related to sarcoglycan complex, laminin complex, cell junction, membrane, adhesion and extracellular matrix was observed among these proteins. We further confirmed the presence of newly identified MTJ proteins by immunofluorescence in human MTJ: Xin actin-binding repeat-containing protein 2 (XIRP2), thrombospondin-4 (THBS4), tetraspanin-24 (CD151), kindlin-2 (FERMT2), cartilage intermediate layer protein 1 (CILP) and integrin-alpha10 (ITGA10). Altogether, these data provide the first detailed proteomics resource of human MTJ.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:54:14.119.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-24 23:58:35	ID requested
1	2022-03-01 00:19:45	announced
⏵ 2	2023-11-14 08:54:14	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: tendon, proteome, myotendinous junction, LC-MS/MS

Atul S Deshmukh
contact affiliation	Novo Nordisk Center For Basic Metabolic Research, University of Copenhagen.
contact email	atul.deshmukh@sund.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD029307

PRIDE project URI

• PRIDE
  1. PXD029307
     1. Label: PRIDE project
     2. Name: Novel tissue-specific markers revealed by in-depth proteome analysis of human myotendinous junction