PXD029288

PXD029288 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomics reveals the temporal phosphorylation network regulated via mesenchymal stem cells in the tumor microenvironment
Description	The tumor microenvironment (TME), which comprises cellular and noncellular components, is involved in the complex process of cancer development. Emerging evidence suggests that mesenchymal stem cells (MSCs), one of the vital regulators of the TME, foster tumor progression through paracrine secretion. However, the comprehensive phospho-signaling pathways that are mediated by MSCs-secreting factors have not yet been fully established. In this study, we attempt to dissect the MSCs-triggered mechanism in lung cancer using quantitative phosphoproteomics. A total of 1958 phosphorylation sites are identified in lung cancer cells stimulated with MSCs-conditioned medium (MSC-CM). Integrative analysis of the identified phosphoproteins and predicted kinases demonstrates that MSC-CM functionally promotes the proliferation and migration of lung cancer via the ERK/phospho-c-Fos-S374 pathway. Recent studies have reported that extracellular ATP accumulates in the tumor microenvironment and stimulates the P2X7 receptor on the cancer cell membrane via purinergic signaling. We observe that ectopic ATP synthase is located on the surface of MSCs and excreted extracellular ATP into the lung cancer microenvironment to trigger the ERK/phospho-c-Fos-Ser374 pathway, which is consistent with these previous findings. Our results suggest that ectopic ATP synthase on the surface of MSCs releases extracellular ATP into the tumor microenvironment, which promotes cancer progression via activation of the ERK/phospho-c-Fos-Ser374 pathway.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_02:58:59.914.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yi-Wen Chang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-22 06:56:55	ID requested
⏵ 1	2022-06-09 02:59:00	announced

Chang YW, Wang CC, Yin CF, Wu CH, Huang HC, Juan HF, Quantitative phosphoproteomics reveals ectopic ATP synthase on mesenchymal stem cells to promote tumor progression via ERK/c-Fos pathway activation. Mol Cell Proteomics, 21(6):100237(2022) [pubmed]

submitter keyword: Phosphoproteomics, Tumor microenvironment, Mesenchymal stem cell, Ectopic ATP synthase, P2X7R, MAPK, c-FOS

Hsueh-Fen Juan
contact affiliation	Department of Life Science, Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan.
contact email	yukijuan@ntu.edu.tw
lab head
Yi-Wen Chang
contact affiliation	Department of Life Science, National Taiwan University
contact email	iwen90322@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029288
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomics reveals the temporal phosphorylation network regulated via mesenchymal stem cells in the tumor microenvironment