PXD029283

PXD029283 is an original dataset announced via ProteomeXchange.

Title	Mapping PP1c and its Inhibitor 2 interactomes reveals conserved and specific networks in asexual and sexual stages of Plasmodium
Description	Malaria parasites require multiple phosphorylation and dephosphorylation steps to drive signaling pathways for proper differentiation and transformation. Several protein phosphatases, including Protein Phosphatase 1 (PP1c), one of the main dephosphorylation enzymes, have been shown to be indispensable for Plasmodium life cycle. PP1c functions are elaborated processes via dynamic interactions with a vast number of binding partners that contribute to its diversity of action. In this study, we have used Plasmodium berghei transgenic parasite strains stably expressing PP1c or its Inhibitor 2 (I2) tagged with mCherry, combined with mCherry affinity pulldown of proteins from asexual and sexual stages and mass spectrometry analyses. Mapped proteins were used to identify interactomes and to cluster functionally related proteins. Our findings confirm previous physical interactions of PP1c and reveal shared and specific biological processes enrichment linked to cellular component assembly in both schizonts and gametocytes, to biosynthetic process/translation in schizonts and to protein transport exclusively in gametocytes. Further, our analysis of PP1c and I2 interactomes reveals that nuclear export mediator factor and peptidyl-prolyl cis-trans isomerase, suggested to be essential in P. falciparum, could be potential targets of the complex PP1c/I2 in both asexual and sexual stages. Our study emphasizes the adaptability of Plasmodium PP1c and provides a fundamental study of the protein interaction landscapes involved in a myriad of events in Plasmodium, suggesting why it is crucial to the parasite and a source for alternative therapeutic strategies.
HostingRepository	PRIDE
AnnounceDate	2022-02-22
AnnouncementXML	Submission_2022-02-22_04:17:22.620.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cerina Chhuon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Plasmodium berghei ANKA; NCBI TaxID: 5823;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-22 06:56:36	ID requested
⏵ 1	2022-02-22 04:17:23	announced

De Witte C, Aliouat EM, Chhuon C, Guerrera IC, Pierrot C, Khalife J, . Int J Mol Sci, 23(3):(2022) [pubmed]

submitter keyword: Interactomics,Protein Phosphatase 1, Inhibitor 2, Plasmodium, Interactome, dephosphorylation, PP1 partners.

Chiara Guerrera
contact affiliation	Proteomics platform Necker, Université de Paris - Structure Fédérative de Recherche Necker, INSERM US24/CNRS UMS3633, Paris, France
contact email	chiara.guerrera@inserm.fr
lab head
Cerina Chhuon
contact affiliation	Proteomics Platform Necker, PPN-3P5, Structure Fédérative de Recherche SFR Necker, Université Paris Descartes, Paris, France
contact email	cerina.chhuon@inserm.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD029283

PRIDE project URI

• PRIDE
  1. PXD029283
     1. Label: PRIDE project
     2. Name: Mapping PP1c and its Inhibitor 2 interactomes reveals conserved and specific networks in asexual and sexual stages of Plasmodium