PXD029277

PXD029277 is an original dataset announced via ProteomeXchange.

Title	SS18/BAFs regulate chromatin accessibility dynamics during pluripotent-somatic transition
Description	The exit from pluripotency or pluripotent-somatic transition (PST) landmarks an event of mammalian development, and is also a representative cell-fate transition model, but remains largely unresolved. Recently, we reported construction of robust JUN-induced PST completed in one cell cycle and whose dominant regulator SS18/BAFs (Brg1/Brahma-associated factors). However, the transition process in the chromatin architecture and the roles played by BAF are still unknown. Here we report the dynamic changes of chromatin accessibility during JUN-induced PST. Meanwhile, SS18/BAFs mediates PST process by relocating from pluripotent loci to AP-1 associated ones and once compromised, JUN fails to open chromatin and PST will be delayed. Furthermore, we show that the relocation of SS18/BAF partially relays on histone modification H3K27ac, instead of JUN-centric protein-protein interaction. These results reveal the orchestration of master transcription factor, epigenetic machine, and histone modification in the cell fate transition.
HostingRepository	iProX
AnnounceDate	2021-10-22
AnnouncementXML	Submission_2022-10-13_23:44:26.822.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ziwei Zhai
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-21 21:51:39	ID requested
1	2021-10-21 21:51:58	announced
⏵ 2	2022-10-13 23:44:27	announced	2022-10-14: Update publication information.

Lin R, Zhai Z, Kuang J, Wu C, Yao Y, Shi R, He J, Xu S, Li P, Fan Y, Li W, Wu Z, Li X, Ming J, Guo J, Wang B, Li D, Cao S, Zhang X, Li Y, Pei D, Liu J, H3K27ac mediated SS18/BAFs relocation regulates JUN induced pluripotent-somatic transition. Cell Biosci, 12(1):89(2022) [pubmed]

submitter keyword: JUN, SS18/BAFs complex, pluripotent-somatic transition, mouse embryonic stem cells, chromatin accessibility, cell fate determination

Jing Liu
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	liu_jing@gibh.ac.cn
lab head
Ziwei Zhai
contact affiliation	Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences
contact email	zhai_ziwei@gibh.ac.cn
dataset submitter

iProX dataset URI