PXD029273

PXD029273 is an original dataset announced via ProteomeXchange.

Title	Proteomic and phosphoproteomic landscape reveals the functional contributions of circadian gene Bmal1 in rat nucleus pulposus cells
Description	Intervertebral disc degeneration (IDD) is a major cause of low back pain (LBP), and the pathogenesis remains unknown. Recently, an autoregulating circadian rhythm was identified in intervertebral discs (IVDs), the abolition of which led to IDD. The genetic disruption of the mouse IVD molecular clock, specifically through the disruption of Bmal1, predisposes mice to IDD. However, the precise role of circadian gene Bmal1 in IDD remain elusive. Using a tandem mass tag (TMT)-based quantitative proteomics approach, we characterized the proteomes and phosphoproteomes of rat nucleus pulposus cells (NPCs) treated with Bmal1 shRNA or its negative control lentivirus.
HostingRepository	iProX
AnnounceDate	2021-10-21
AnnouncementXML	Submission_2023-12-21_20:45:08.491.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liangwei Mei
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	TMT6plex reporter fragment
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-21 19:53:39	ID requested
⏵ 1	2023-12-21 20:45:09	announced

Mei L, Zheng Y, Gao X, Ma T, Xia B, Hao Y, Wei B, Wei Y, Luo Z, Huang J, Hsa-let-7f-1-3p targeting the circadian gene Bmal1 mediates intervertebral disc degeneration by regulating autophagy. Pharmacol Res, 186():106537(2022) [pubmed]

submitter keyword: Bmal1, circadian rhythm, intervertebral disc degeneration, nucleus pulposus

Jinghui Huang
contact affiliation	Xijing Hospital, the Fourth Military Medical University
contact email	huangjh@fmmu.edu.cn
lab head
Liangwei Mei
contact affiliation	Xijing Hospital, the Fourth Military Medical University
contact email	mliangwei@hotmail.com
dataset submitter

iProX dataset URI