PXD029266

PXD029266 is an original dataset announced via ProteomeXchange.

Title	MATN2 knock-down human immortalized podocytes LC-MS/MS
Description	Basement membranes (BMs) are ubiquitous extracellular matrices whose composition remains elusive, limiting our understanding of BM regulation and function. By developing a bioinformatic and in vivo discovery pipeline, we define a network of over 220 human proteins localized to BMs. More than 100 BM-network genes associate with human phenotypes and by screening over 63,000 germline genomic sequences from families with rare disorders, we discovered novel predicted loss-of-function variants in MATN2. Biochemical analyses suggest that MATN2 interacts with many BM proteins, and we found that depletion of MATN2 affected levels of core BM components in human podocyte-derived extracellular matrix.
HostingRepository	PRIDE
AnnounceDate	2022-08-01
AnnouncementXML	Submission_2022-08-01_02:46:33.201.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mychel Morais
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-21 05:08:20	ID requested
1	2022-05-31 03:59:49	announced
⏵ 2	2022-08-01 02:46:34	announced	2022-08-01: Updated project metadata.

Jayadev R, Morais MRPT, Ellingford JM, Srinivasan S, Naylor RW, Lawless C, Li AS, Ingham JF, Hastie E, Chi Q, Fresquet M, Koudis NM, Thomas HB, O'Keefe RT, Williams E, Adamson A, Stuart HM, Banka S, Smedley D, Sherwood DR, Lennon R, A basement membrane discovery pipeline uncovers network complexity, regulators, and human disease associations. Sci Adv, 8(20):eabn2265(2022) [pubmed]

submitter keyword: Q Exactive HF, podocyte, human, Shotgun mass spectrometry,MATN2

Racehl Lennon
contact affiliation	Wellcome Centre for Cell-Matrix Research, Division of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, Faculty of Biology Medicine and Health, The University of Manchester, United Kingdom
contact email	rachel.lennon@manchester.ac.uk
lab head
Mychel Morais
contact affiliation	The University of Manchester
contact email	mychel.morais@manchester.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD029266

PRIDE project URI

• PRIDE
  1. PXD029266
     1. Label: PRIDE project
     2. Name: MATN2 knock-down human immortalized podocytes LC-MS/MS