Updated project metadata. It is known that exogenous factors influences sperm quality and function, such as toxins, exercise, diet and smoking. Still, the moleuclar mechanisms of this is not understood. Here, we set out to determine how a dietary insult of different sugar levels in the diet affects Drosophila sperm proteome, by dissecting out seminal vesicles containing mature sperm from adult flies. These flies have been on a dietary intervention of two days on a sugar diet of 3, 30 or 300 g/L of sugar. Whole seminal vesicles were then used for mass spectometry to increase our understanding of how an acute dietary intervention may change sperm proteomics.