PXD029263

PXD029263 is an original dataset announced via ProteomeXchange.

Title	Differential impact of phosphate availability on primary and specialized metabolism of Streptomyces lividans and Streptomyces coelicolor.
Description	In order to define the impact of phosphate (Pi) availability on cellular metabolism the project aimed to perform a comparative analysis of the proteomes of two Streptomyces strains with different abilities to produce antibiotics, S. coelicolor and S. lividans as well as of the pptA mutant of S. lividans, grown low (1mM) and high (5mM) phosphate (Pi) availability conditions. Interestingly, in contrast to most Streptomyces species, S. coelicolor produces more antibiotics in Pi proficiency than in Pi limitation, S. lividans does not produce antibiotics in any Pi conditions and the pptA mutant produces antibiotics only in Pi limitation. This in-depth proteomic comparison of three Streptomyces strains (S. coelicolor, S. lividans wt and pptA mutant), in different growth conditions (time and Pi concentration in the medium) was performed on four biological replicates. Protein abundance changes were determined using two label-free mass spectrometry based-quantification methods: spectral count (SC) and MS1 ion intensities named XIC (for eXtracted Ion Current). Our proteomic data reveal for the first time, the impact of Pi availability on the abundance of approximately 4000 proteins of these Streptomyces strains with different abilities to produce antibiotics. The most striking feature differentiating these strains was the much higher abundance of enzymes of the respiratory chain in both phosphate conditions in S. coelicolor compared to the S. lividans strains.
HostingRepository	PRIDE
AnnounceDate	2022-02-04
AnnouncementXML	Submission_2022-02-04_03:48:11.748.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD029263
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Cornu
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902; scientific name: Streptomyces lividans TK24; NCBI TaxID: 457428;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-21 05:07:28	ID requested
⏵ 1	2022-02-04 03:48:12	announced

Dataset with its publication pending

submitter keyword: Streptomyces, proteomics, label-free protein quantification, central carbon metabolism, phosphate, respiratory chain, oxidative stress, antibiotics,

Marie-Joelle Virolle
contact affiliation	Université Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198, Gif-sur-Yvette, France.
contact email	marie-joelle.virolle@i2bc.paris-saclay.fr
lab head
David Cornu
contact affiliation	SICaPS, Institute of Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Univ. Paris-Saclay 91198 Gif-sur-Yvette Cedex, France
contact email	david.cornu@i2bc.paris-saclay.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029263
     1. Label: PRIDE project
     2. Name: Differential impact of phosphate availability on primary and specialized metabolism of Streptomyces lividans and Streptomyces coelicolor.