PXD029260

PXD029260 is an original dataset announced via ProteomeXchange.

Title	A Redox-Dependent Pathway for Regulating Reversible HDACs Synchronizes Crown Root Development in Rice
Description	As a signal molecular in aerobic organism, how reactive oxygen species (ROS) regulates normal life is a fundamental biological question. Locally accumulated ROS have been reported to balance cell division and differentiation in root apical meristem. However, the underlying molecular mechanism is unclear. Here, we reveal that developmentally produced H2O2 in plant root apical meristem (RAM) triggers reversible acetylation modification of proteins which involved in protein synthesis and cell proliferation. WOX11, an essential transcription factor for crown roots (CRs) formation, modulates ROS homeostasis by directly regulating class III peroxidases. HDACs (histone deacetylases) sense cellular redox status to enhance their enzyme activities, which drives protein acetylation level alterations in rice roots. Oxidation-dependent on WOX11 triggered protein acetylation modification leads to a robust root system in rice. Our study revealed a novel regulatory mechanism which cellular redox status via a WOX11-dependent manner regulates protein acetylation during rice crown root development. The molecular link between the redox status and HDACs activities through WOX11-dependent pathway may provide new insight into which plants exploit developmentally produced ROS to direct organogenesis. Additionally, HDACs reversible enzyme activity via redox-regulated endows with the flexibility of protein modification control in dealing with developmental and changing environmental cues.
HostingRepository	PRIDE
AnnounceDate	2022-08-10
AnnouncementXML	Submission_2022-08-10_07:05:07.268.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	qiutao xu
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 23:17:51	ID requested
⏵ 1	2022-08-10 07:05:08	announced

Xu Q, Wang Y, Chen Z, Yue Y, Huang H, Wu B, Liu Y, Zhou DX, Zhao Y, ROS-stimulated protein lysine acetylation is required for crown root development in rice. J Adv Res, 48():33-46(2023) [pubmed]

submitter keyword: Acetylome；ROS；Rice root；HDAC；WOX11

Qiutao Xu
contact affiliation	Huazhong Agricultural University
contact email	skyyks123@webmail.hzau.edu.cn
lab head
qiutao xu
contact affiliation	Huazhong Agricultural University
contact email	skyyks123@webmail.hzau.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029260
     1. Label: PRIDE project
     2. Name: A Redox-Dependent Pathway for Regulating Reversible HDACs Synchronizes Crown Root Development in Rice