PXD029250

PXD029250 is an original dataset announced via ProteomeXchange.

Title	The proteomic response of Clostridioides difficile to the atypical tetracycline amidochelocardin and concomitant dissipation of its proton motive force
Description	Amidochelocardin is an atypical tetracycline which was previously shown to target the bacterial cell membrane although its detailed mode-of-action is still unknown. To gain insight into Clostridioides difficile’s response to membrane targeting antibiotics and to further study the mode-of-action of Amidochelocardin, the stress response of C. difficile 630 to sublethal doses of amidochelocardin were studied on the proteome level. The results and results from downstream experiments, such as quantification of the membrane potential, cellular localization assays and transmission electron microscopy analysis, suggest that amidochelocardin kills bacteria such as C. difficile by dissipating their membrane potential. Furthermore, the data suggest that C. difficile responds to dissipation of the membrane potential by induction of biosynthesis of an hitherto unknown aromatic compound.
HostingRepository	PRIDE
AnnounceDate	2022-08-04
AnnouncementXML	Submission_2022-08-04_07:54:56.038.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Clostridioides difficile 630; NCBI TaxID: 272563;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 08:13:20	ID requested
⏵ 1	2022-08-04 07:54:56	announced

Dataset with its publication pending

submitter keyword: amidochelocardin, Clostridioides difficile, antibiotic therapy, antibiotic resistance, dissipation of the proton motive force, tetracycline, phenazine biosynthesis

Susanne Sievers
contact affiliation	University of Greifswald, Institute of Microbiology, Department of Microbial Physiology and Molecular Biology
contact email	sieverss@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	University of Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD029250

PRIDE project URI

• PRIDE
  1. PXD029250
     1. Label: PRIDE project
     2. Name: The proteomic response of Clostridioides difficile to the atypical tetracycline amidochelocardin and concomitant dissipation of its proton motive force