PXD029242

PXD029242 is an original dataset announced via ProteomeXchange.

Title	Definition and characterization of the mitochondrial proteome of human cells (MitoCoP): Relation between MS analysis time, sample fractionation, proteases, replicates and number of proteins identified/quantified
Description	Aim of the overall project was the definition and characterization of the mitochondrial proteome of human cells, referred to as MitoCoP. To this end, we combined the complementary strengths of subtractive proteomics, ImportOmics and subcellular protein profiling in a global multidimensional classification approach. In this part of the project, we analyzed the correlation between MS analysis time, sample fractionation, use of multiple proteases and replicates on the number of proteins identified and quantified in mitochondrial fractions applying the subtractive proteomics approach.
HostingRepository	PRIDE
AnnounceDate	2021-11-25
AnnouncementXML	Submission_2021-11-25_08:31:24.375.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Friedel Drepper
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 03:06:29	ID requested
⏵ 1	2021-11-25 08:31:24	announced

Dataset with its publication pending

submitter keyword: subtractive proteomics, mitochondria, SILAC

Prof. Dr. Bettina Warscheid
contact affiliation	Institute of Biology II, Biochemistry – Functional Proteomics, Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany CIBSS Centre for Integrative Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany Department of Microbiology, Biocenter, University of Würzburg, 97074 Würzburg, Germany
contact email	Bettina.Warscheid@biologie.uni-freiburg.de
lab head
Friedel Drepper
contact affiliation	AG Warscheid Biologie II Albert-Ludwigs-Universität Freiburg Schänzlestr. 1 79104 Freiburg Germany
contact email	friedel.drepper@biologie.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD029242

PRIDE project URI

• PRIDE
  1. PXD029242
     1. Label: PRIDE project
     2. Name: Definition and characterization of the mitochondrial proteome of human cells (MitoCoP): Relation between MS analysis time, sample fractionation, proteases, replicates and number of proteins identified/quantified