PXD029239

PXD029239 is an original dataset announced via ProteomeXchange.

Title	Acacia Catechu Willd extract protects neuronal cells from oxidative stress-induced damage
Description	Oxidative stress (OS) resulting in reactive oxygen species (ROS) generation and inflammation, play a pivotal role in the neuronal loss occurring in neurodegenerative diseases. Therefore, promising future drugs that would prevent or slow down the progression of neurodegeneration should possess potent radical-scavenging activity. Acacia catechu Willd heartwood extract (AC) contains high amount of catechins endowed of antioxidant properties. The aim of the present study was to assess AC neuroprotection in rat brain slices treated with hydrogen peroxide. AC reverted the decrease in viability as well as the increase in sub-diploid-, DAPI positive cells, reduced ROS formation, recovered the mitochondrial potential and caspase-3 activation. Neuroprotective effects occurred in rat brain slices as a reversal in the expression of the main proteins involved in apoptosis and signalling pathways related to calcium homeostasis following OS-mediated injury was demonstrated. Additionally, unbiased quantitative mass spectrometry allowed us to assess that AC partially reverted the hydrogen peroxide-induced altered proteome, including proteins belonging to the synaptic vesicle fusion apparatus. In conclusion, the present results suggest the possibility of AC as a nutraceutical useful in preventing neurodegenerative diseases.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_08:15:13.637.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roberto Stella
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 03:06:09	ID requested
⏵ 1	2022-02-16 08:15:14	announced

Chiaino E, Stella R, Peggion C, Micucci M, Budriesi R, Mattioli LB, Marzetti C, Pessina F, Valoti M, Frosini M, Willd. Extract Protects Neuronal Cells from Oxidative Stress-Induced Damage. Antioxidants (Basel), 11(1):(2021) [pubmed]

submitter keyword: Brain, Rat, Acacia Catechu, LC-HRMS/MS

Maria Frosini
contact affiliation	Department of Life Sciences, University of Siena, Italy
contact email	maria.frosini@unisi.it
lab head
Roberto Stella
contact affiliation	Istituto Zooprofilattico Sperimentale delle Venezie
contact email	rstella@izsvenezie.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029239
     1. Label: PRIDE project
     2. Name: Acacia Catechu Willd extract protects neuronal cells from oxidative stress-induced damage