PXD029234

PXD029234 is an original dataset announced via ProteomeXchange.

Title	Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4,6,8
Description	Plants are able to sense changes in their light environments, such as the onset of day and night, as well as anticipate these changes in order to adapt and survive. Central to this ability is the plant circadian clock, a molecular circuit that precisely orchestrates plant cell processes over the course of a day. REVEILLE proteins (RVEs) are recently discovered members of the plant circadian circuitry that activate the evening complex and PRR genes to maintain regular circadian oscillation. The RVE4, 6 and 8 proteins in particular have been shown to limit the length of the circadian period, with rve4/6/8 plants possessing an elongated period as well as increased leaf surface area and biomass relative to wild-type Col-0 plants. Here, using a multi-omics approach consisting of phenomics, transcriptomics, proteomics, and metabolomics we demonstrate how RVE8-like proteins impact diel plant cell function and draw novel connections to a number of plant cell processes that underpin the growth and development phenotypes observed in rve4/6/8 plants. In particular, we reveal that loss of RVE8-like proteins results in altered carbohydrate and lipid metabolism, including a starch excess phenotype at ZT0. We further demonstrate that RVE8-like proteins has an unique impact on the abundance and phosphorylation 26S proteasome subunits, in addition to impacting the abundance and/or phosphorylation status of a number of protein kinases. . Overall, this robust, multi-omic dataset provides substantial new molecular insights into RVE8-like protein function demonstrating the far reaching impact RVE8-like proteins have on the diel plant cell environment.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_03:45:03.362.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Uhrig
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-20 03:05:39	ID requested
⏵ 1	2021-11-03 03:45:03	announced

Dataset with its publication pending

submitter keyword: arabidopsis, circadian clock, reveille, boxcarDIA, phosphoproteome

R. Glen Uhrig
contact affiliation	Department of Biological Sciences, University of Alberta
contact email	ruhrig@ualberta.ca
lab head
Richard Uhrig
contact affiliation	University of Alberta
contact email	ruhrig@ualberta.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD029234
     1. Label: PRIDE project
     2. Name: Multi-omic analysis reveals the biochemical changes underpinning the varied phenotypes of the Arabidopsis long-period mutant rve 4,6,8