PXD029222

PXD029222 is an original dataset announced via ProteomeXchange.

Title	Listeria monocytogenes responded to dehydrocorydaline
Description	Listeria monocytogenes is a foodborne pathogen, with relatively low incidence but high case-fatality. Phytochemicals have been recognized as a promising antimicrobial agent as an alternative to synthetic chemicals due to their safety and high efficacy with multi-target sites. This study identified and characterized a novel antibacterial agent, dehydrocorydaline, in Corydalis turschaninovii rhizome using HPLC-LTQ-Orbitrap-HRMS and its antibacterial effect with lowest MIC (1 mg/mL) and MBC (2 mg/mL) values. In addition, in vitro growth kinetic assay, cytoplasmic nucleic acid and protein leakage assay and observation of morphological changes in bacterial cells supported the strong antibacterial activity. Herein, quantitative proteomic analysis was performed to investigate the molecular mechanism of antibacterial action of dehydrocorydaline against L. monocytogenes. Taking together with proteomic results, we found that dehydrocorydaline influenced on the global proteomic alteration of L. monocytogenesis associated with carbohydrate metabolism, energy metabolism, cell wall synthesis, and bacterial motility, suggesting multiple target sites of dehydrocorydaline to inactivate bacterial growth. This study demonstrated that dehydrocorydaline has potential as a natural and effective antibacterial agent with multi-target sites in pathogenic bacteria, and provides the basis for development of a new class of antibacterial agent.
HostingRepository	iProX
AnnounceDate	2021-10-19
AnnouncementXML	Submission_2022-10-13_21:09:24.886.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gowoon Kim
SpeciesList	scientific name: Listeria monocytogenes EGD-e; NCBI TaxID: 169963; scientific name: Listeria monocytogenes EGD; NCBI TaxID: 1334565;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-10-19 23:59:52	ID requested
1	2021-10-20 00:01:32	announced
⏵ 2	2022-10-13 21:09:25	announced	2022-10-14: Update publication information.

Kim G, Xu Y, Zhang J, Sui Z, Corke H, . Front Microbiol, 12():799094(2021) [pubmed]

submitter keyword: antibacterial mechanism of action of dehydrocorydaline

Zhongquan Sui
contact affiliation	Shanghai Jiao Tong University
contact email	zsui@sjtu.edu.cn
lab head
Gowoon Kim
contact affiliation	Shanghai Jiao Tong University
contact email	gowoon_kim@sjtu.edu.cn
dataset submitter

iProX dataset URI